Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99.
Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99.
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猪流行腹泻病毒Chinju99的尖峰基因的克隆和序列分析。
DOI：
10.1023/a:1024443112717
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发表时间：
2003-05
期刊：
影响因子：
1.6
通讯作者：
Nagy EE
中科院分区：
文献类型：
--
作者：
Yeo SG;Hernandez M;Krell PJ;Nagy EE
为研制猪流行性腹泻病毒基因工程疫苗和诊断试剂，克隆了猪流行性腹泻病毒原分离株中国株99株的尖峰蛋白(S)基因，并进行了序列测定。编码锦桔99株S基因开放阅读框的核苷酸序列全长4152个碱基，编码1383个氨基酸。其中腺嘌呤1001个(24.1%)，胞嘧啶849个(20.4%)，鸟嘌呤877个(21.1%)，胸腺嘧啶1425个(34.3%)。结果表明，该序列与Br1/87株和CV777株的核苷酸同源性分别为94.5%和94.5%。该蛋白与Br1/87和CV777的氨基酸同源性分别为92.8%和92.8%。该氨基酸序列包含27个可能的天冬酰胺(N)连接的糖基化位点，在第1325-1350位有一段高度疏水的残基。
The spike (S) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to aid in the development of genetically engineered vaccines and diagnostic reagents against PEDV. The nucleotide sequence encoding the entire S gene open reading frame (ORF) of Chinju99 was 4152 bases long encoding 1383 amino acids. It consisted of 1001 adenine (24.1%), 849 cytosine (20.4%), 877 guanine (21.1%) and 1425 thymine (34.3%) residues. The Chinju99 S ORF nucleotide sequence was 94.5% homologous with that of the Br1/87 and CV777 strains, respectively. The Chinju99 S protein had 92.8% amino acid identity with that of Br1/87 and CV777, respectively. The amino acid sequence contained 27 potential sites for asparagine (N)-linked glycosylation and there was a stretch of highly hydrophobic residues at position 1325–1350.
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影响因子：
3.7
作者：
DUARTE, M;TOBLER, K;LAUDE, H
通讯作者：
LAUDE, H
影响因子：
3.3
作者：
Knuchel M;Ackermann M;Müller HK;Kihm U
通讯作者：
Kihm U
猪腹泻冠状病毒（PEDV）基因组序列的完成。
影响因子：
1.6
作者：
Kocherhans R;Bridgen A;Ackermann M;Tobler K
通讯作者：
Tobler K
影响因子：
2.4
作者：
Chasey D;Cartwright SF
通讯作者：
Cartwright SF
影响因子：
3.8
作者：
DUARTE, M;LAUDE, H
通讯作者：
LAUDE, H