Molecular detection of parasitic protozoa

Molecular detection of parasitic protozoa
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寄生原虫的分子检测

DOI:
10.1017/s0031182099004102
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发表时间:
1999
期刊:
影响因子:
2.4
通讯作者:
R. Thompson
R. Thompson
中科院分区:
医学2区
文献类型:
--
作者:
U. Morgan;R. Thompson

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分子诊断方法的发展,特别是利用 PCR 检测寄生原生动物的方法,将通过提高诊断速度、特异性和敏感性、再现性和易于解释性,极大地促进这些病原体的识别和控制。然而,PCR 方法并非没有问题,在基于 PCR 的检测被接受为原生动物寄生虫常规检测的首选工具之前,需要改进实验室程序。讨论了PCR检测在各种寄生虫中的应用。
The development of molecular diagnostic methods, particularly those utilizing PCR for the detection of parasitic protozoa will contribute greatly to the identification and control of these pathogens, by increasing speed of diagnosis, specificity and sensitivity, reproducibility and ease of interpretation. PCR methods are not without their problems however, and there is a need for laboratory procedures to be refined before PCR-based assays are accepted as the tools of choice for the routine detection of protozoan parasites. The application of PCR detection to various parasites is discussed.
通过聚合酶链反应和原位杂交检测受感染的小鼠心脏组织中的克氏锥虫。
DOI: 10.4269/ajtmh.1997.56.588
发表时间: 1997
期刊: The American journal of tropical medicine and hygiene
影响因子: --
作者:
Lane,JE;Olivares-Villagomez,D;Vnencak-Jones,CL;McCurley,TL;Carter,CE
通讯作者: Carter,CE
DOI: 10.1021/ac960718q
发表时间: 1996-12-01
影响因子: 7.4
作者:
Woolley, AT;Hadley, D;Northrup, MA
通讯作者: Northrup, MA