Prognostic analysis of invasive circulating tumor cells (iCTCs) in epithelial ovarian cancer.
Prognostic analysis of invasive circulating tumor cells (iCTCs) in epithelial ovarian cancer.
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上皮性卵巢癌侵袭性循环肿瘤细胞（iCTC）的预后分析。
DOI：
10.1016/j.ygyno.2014.06.013
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发表时间：
2014-09
影响因子：
4.7
通讯作者：
Chen WT
中科院分区：
文献类型：
--
作者：
Pearl ML;Zhao Q;Yang J;Dong H;Tulley S;Zhang Q;Golightly M;Zucker S;Chen WT
循环肿瘤细胞（CTC）已被引入作为检测晚期上皮性卵巢癌（EOC）的生物标志物。目的是检查EOC高风险患者中浸润性CTC亚群（iCTC）的患病率，并将该生物标志物与血清CA 125进行比较。我们使用独特的基于细胞粘附基质（CAM）的功能性细胞富集和鉴定平台，从129名术前患者中分离iCTC。我们使用阳性上皮（Epi+）标志物和阴性造血谱系（HL-）标志物确认了iCTC的身份。检测了检测的灵敏度和特异性，并将iCTC/CA 125与总生存期（OS）、无进展生存期（PFS）和临床参数相关。我们发现iCTC检测在检测I期和II期EOC恶性肿瘤患者中的灵敏度为41.2%，特异性为95.1%，阳性预测值（PPV）为77.8%，在检测所有阶段的EOC恶性肿瘤中的灵敏度为83%，PPV为97.3%。然而，阳性CA 125检测提供了弱证据检测I和II期恶性肿瘤（61.6%PPV）和所有EOC（92.1%PPV），因为其特异性为76.2%。与血清CA 125相比，iCTC升高的临床因素（肿瘤分期、减瘤和铂敏感性）的OS和PFS具有显著更强的一致性。CAM启动的CTC富集/鉴定方法能够检测早期EOC。iCTC与更差的OS和PFS相关性更好，在检测EOC高风险患者的EOC恶性肿瘤方面，其PPV比CA 125更具特异性和更好。
Circulating tumor cells (CTCs) have been introduced as a biomarker in detecting advanced Epithelial Ovarian Cancer (EOC). The goals are to examine the prevalence of the invasive subpopulation of CTCs (iCTCs) in patients at high risk of EOC and to compare this biomarker to serum CA125. We used a unique Cell Adhesion Matrix (CAM)-based, functional cell enrichment and identification platform to isolate iCTCs from 129 preoperative patients. We confirmed the identity of iCTCs using positive epithelial (Epi+) markers and negative hematopoietic lineage (HL-) markers. Sensitivity and specificity of the assays were examined and iCTCs / CA125 were correlated with overall survival (OS), progression-free survival (PFS) and clinical parameters. We found a 41.2% sensitivity, 95.1% specificity and 77.8% positive predictive value (PPV) of the iCTC assay in detecting patients with stage I and II EOC malignancy, and a 83% sensitivity and 97.3% PPV in detecting all stages of EOC malignancy. However, a positive CA125 test provided weak evidence to detect stage I and II malignancy (61.6% PPV) and all EOC (92.1% PPV), because of its 76.2% specificity. A significantly stronger concordance in OS and PFS of clinical factors (tumor stage, debulking and platinum sensitivity) was noted for elevated iCTCs than for serum CA125. The CAM-initiated CTC enrichment / identification method enabled the detection of early stage EOC. iCTCs were better correlated with worse OS and PFS, more specific and better PPV than CA125 in detecting EOC malignancy in patients at high risk of EOC.
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DOI：
10.1186/bcr2131
发表时间：
2008
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Deng G;Herrler M;Burgess D;Manna E;Krag D;Burke JF
通讯作者：
Burke JF
影响因子：
4.8
作者：
Nassar, Dany;Droitcourt, Catherine;Aractingi, Selim
通讯作者：
Aractingi, Selim
影响因子：
4.7
作者：
Levy, Tally;Weiser, Roi;Menczer, Joseph
通讯作者：
Menczer, Joseph
DOI：
10.1097/igc.0b013e318216cb91
发表时间：
2011-07-01
影响因子：
4.8
作者：
Aktas, Bahriye;Kasimir-Bauer, Sabine;Wimberger, Pauline
通讯作者：
Wimberger, Pauline
DOI：
10.1097/igc.0b013e3182691254
发表时间：
2012-10-01
影响因子：
4.8
作者：
Levy, Tally;Weiser, Roi;Menczer, Joseph
通讯作者：
Menczer, Joseph