EgMIXTA1, a MYB-Type Transcription Factor, Promotes Cuticular Wax Formation in Eustoma grandiflorum Leaves.
EgMIXTA1, a MYB-Type Transcription Factor, Promotes Cuticular Wax Formation in Eustoma grandiflorum Leaves.
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MYB 型转录因子 EgMIXTA1 促进洋桔梗叶角质层蜡质形成
DOI：
10.3389/fpls.2020.524947
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发表时间：
2020
影响因子：
5.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Wang L;Xue W;Li X;Li J;Wu J;Xie L;Kawabata S;Li Y;Zhang Y
在气生植物器官中，表皮蜡形成疏水层，可以保护细胞免受脱水，抵御病原体攻击，并防止器官在发育过程中融合。MIXTA基因编码MYB样转录因子，其与表皮蜡质生物合成相关以增加叶片表面的蜡质负载。在这项研究中，AmMIXTA同源基因EgMIXTA 1的功能特点，在洋桔梗。EgMIXTA 1在叶和芽中普遍但高度表达。我们鉴定了洋桔梗MIXTA同源物并开发了用于过表达的植物。EgMIXTA 1-过表达的植物有更多的蜡晶体沉积在叶表面相比，野生型和相当多的整体角质层蜡。在过量表达株系的叶片中，角质层蒸腾速率比非转基因植株的慢。对基因表达的分析表明，已知参与蜡生物合成的几个基因，如EgCER 3、EgCER 6、EgCER 10、EgKCS 1、EgKCR 1和EgCYP 77 A6，被EgMIXTA 1过表达系诱导。另一个基因WAX INDUCER 1/SHINE 1（编码刺激角质产生的转录因子）的表达在过表达者中也显著高于野生型。然而，脂质相关基因EgABCG 12的表达相对于野生型没有变化。这些结果表明EgMIXTA 1参与表皮蜡的生物合成。
In the aerial plant organs, cuticular wax forms a hydrophobic layer that can protect cells from dehydration, repel pathogen attacks, and prevent organ fusion during development. The MIXTA gene encodes an MYB-like transcription factor, which is associated with epicuticular wax biosynthesis to increase the wax load on the surface of leaves. In this study, the AmMIXTA-homologous gene EgMIXTA1 was functionally characterized in the Eustoma grandiflorum. EgMIXTA1 was ubiquitously, but highly, expressed in leaves and buds. We identified the Eustoma MIXTA homolog and developed the plants for overexpression. EgMIXTA1-overexpressing plants had more wax crystal deposition on the leaf surface compared to wild-type and considerably more overall cuticular wax. In the leaves of the overexpression line, the cuticular transpiration occurred more slowly than in those of non-transgenic plants. Analysis of gene expression indicated that several genes, such as EgCER3, EgCER6, EgCER10, EgKCS1, EgKCR1, and EgCYP77A6, which are known to be involved in wax biosynthesis, were induced by EgMIXTA1-overexpression lines. Expression of another gene, WAX INDUCER1/SHINE1, encoding a transcription factor that stimulates the production of cutin, was also significantly higher in the overexpressors than in wild-type. However, the expression of a lipid-related gene, EgABCG12, did not change relative to the wild-type. These results suggest that EgMIXTA1 is involved in the biosynthesis of cuticular waxes.
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DOI：
10.1073/pnas.0706984104
发表时间：
2007-11-13
影响因子：
11.1
作者：
Li, Yonghua;Beisson, Fred;Ohlrogge, John
通讯作者：
Ohlrogge, John
影响因子：
11.6
作者：
Go, Young Sam;Kim, Hyojin;Suh, Mi Chung
通讯作者：
Suh, Mi Chung
影响因子：
7.4
作者：
Beaudoin, Frederic;Wu, Xianzhong;Kunst, Ljerka
通讯作者：
Kunst, Ljerka
影响因子：
7.4
作者：
Hooker, TS;Millar, AA;Kunst, L
通讯作者：
Kunst, L
影响因子：
27.5
作者：
Lee, Saet Buyl;Suh, Mi Chung
通讯作者：
Suh, Mi Chung