Hollow hydroxyapatite microspheres as a device for controlled delivery of proteins.
Hollow hydroxyapatite microspheres as a device for controlled delivery of proteins.
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DOI：
10.1007/s10856-011-4250-6
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发表时间：
2011-03
影响因子：
3.7
通讯作者：
Brown, Roger F.
中科院分区：
文献类型：
--
作者：
Fu, Hailuo;Rahaman, Mohamed N.;Day, Delbert E.;Brown, Roger F.
以Li_2 O-CaO-B_2O_3玻璃微球（106-150 μm）为原料，在磷酸氢二钾（K_2HPO_4）溶液中制备了中空羟基磷灰石（HA）微球，并对模型蛋白牛血清白蛋白（BSA）进行了控释研究。玻璃微球在0.02M K2 HPO 4溶液（pH = 9）中在37°C下反应2天导致形成生物相容性HA微球，其具有等于0.6外径的中空芯直径、高表面积（~100 m2/g）和中孔壳壁（孔径约13 nm）。在用BSA在磷酸盐缓冲盐水（PBS）中的溶液（5 mg BSA/ml）加载后，使用微量二喹啉甲酸（BCA）蛋白质测定来测量BSA从HA微球到PBS介质中的释放动力学。BSA的释放最初随时间线性增加，但在24-48 h后几乎停止。BSA释放动力学的改变通过使用受控的热处理（在600-900°C下1-24小时）改变所制备的HA微球的微结构来实现。在600°C加热5 h后，HA微球可在7-14 d内持续释放BSA。在给定时间释放的BSA的量取决于最初加载到HA微球中的BSA的浓度。这些空心HA微球可以提供一种新的无机装置，用于蛋白质和药物的控制性局部递送。
Hollow hydroxyapatite (HA) microspheres were prepared by reacting solid microspheres of Li2O–CaO–B2O3 glass (106–150 μm) in K2HPO4 solution, and evaluated as a controlled delivery device for a model protein, bovine serum albumin (BSA). Reaction of the glass microspheres for 2 days in 0.02 M K2HPO4 solution (pH = 9) at 37°C resulted in the formation of biocompatible HA microspheres with a hollow core diameter equal to 0.6 the external diameter, high surface area (~100 m2/g), and a mesoporous shell wall (pore size ≈13 nm). After loading with a solution of BSA in phosphate-buffered saline (PBS) (5 mg BSA/ml), the release kinetics of BSA from the HA microspheres into a PBS medium were measured using a micro bicinchoninic acid (BCA) protein assay. Release of BSA initially increased linearly with time, but almost ceased after 24–48 h. Modification of the BSA release kinetics was achieved by modifying the microstructure of the as-prepared HA microspheres using a controlled heat treatment (1–24 h at 600–900°C). Sustained release of BSA was achieved over 7–14 days from HA microspheres heated for 5 h at 600°C. The amount of BSA released at a given time was dependent on the concentration of BSA initially loaded into the HA microspheres. These hollow HA microspheres could provide a novel inorganic device for controlled local delivery of proteins and drugs.
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影响因子：
3.6
作者：
ONO, I;OHURA, T;KUBOKI, Y
通讯作者：
KUBOKI, Y
DOI：
10.4028/www.scientific.net/kem.309-311.65
发表时间：
2006-01-01
期刊：
BIOCERAMICS 18, PTS 1 AND 2
影响因子：
--
作者：
Peng, Q;Ming, L;Weng, J
通讯作者：
Weng, J
影响因子：
3.5
作者：
VERHOEF, AH;DENHARTOG, HW
通讯作者：
DENHARTOG, HW
影响因子：
3.5
作者：
VERHOEF, AH;DENHARTOG, HW
通讯作者：
DENHARTOG, HW
影响因子：
3.6
作者：
RIPAMONTI, U;MA, SS;REDDI, AH
通讯作者：
REDDI, AH