Dietary Factors Modulate Iron Uptake in Caco-2 Cells from an Iron Ingot Used as a Home Fortificant to Prevent Iron Deficiency.
Dietary Factors Modulate Iron Uptake in Caco-2 Cells from an Iron Ingot Used as a Home Fortificant to Prevent Iron Deficiency.
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DOI：
10.3390/nu9091005
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发表时间：
2017-09-12
期刊：
影响因子：
5.9
通讯作者：
Fairweather-Tait SJ
中科院分区：
文献类型：
--
作者：
Rodriguez-Ramiro I;Perfecto A;Fairweather-Tait SJ
缺铁是一个主要的公共卫生问题，需要营养方法来降低其患病率。本研究的目的是检查一种新型家用强化剂“Lucky Iron Fish™”（LIF）（，圭尔夫，加拿大）的铁生物利用度，以及饮食因素和食物基质对Caco-2细胞从LIF摄取铁的影响。LIF释放大量的铁（约1.2 mM），在pH 2，但这种铁仅微溶于pH 7，不采取由细胞。抗坏血酸（AA）的加入保持了从LIF（LIF-铁）在pH 7释放的铁的溶解度，并以浓度依赖性的方式促进细胞对铁的摄取。在体外消化的LIF铁的存在下，豌豆增加铁的吸收10倍。然而，添加单宁酸的消化减少了细胞铁的吸收7.5倍。此外，LIF-铁诱导的活性氧（ROS）的过度生产，类似于硫酸亚铁，但这种效果被抵消了添加AA。总体而言，我们的数据说明了饮食因素对铁的溶解度和LIF的生物利用度的主要影响，并证明了AA的加入增强了铁的吸收并减少了肠腔中的ROS。
Iron deficiency is a major public health concern and nutritional approaches are required to reduce its prevalence. The aim of this study was to examine the iron bioavailability of a novel home fortificant, the “Lucky Iron Fish™” (LIF) (, Guelph, Canada) and the impact of dietary factors and a food matrix on iron uptake from LIF in Caco-2 cells. LIF released a substantial quantity of iron (about 1.2 mM) at pH 2 but this iron was only slightly soluble at pH 7 and not taken up by cells. The addition of ascorbic acid (AA) maintained the solubility of iron released from LIF (LIF-iron) at pH 7 and facilitated iron uptake by the cells in a concentration-dependent manner. In vitro digestion of LIF-iron in the presence of peas increased iron uptake 10-fold. However, the addition of tannic acid to the digestion reduced the cellular iron uptake 7.5-fold. Additionally, LIF-iron induced an overproduction of reactive oxygen species (ROS), similar to ferrous sulfate, but this effect was counteracted by the addition of AA. Overall, our data illustrate the major influence of dietary factors on iron solubility and bioavailability from LIF, and demonstrate that the addition of AA enhances iron uptake and reduces ROS in the intestinal lumen.
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影响因子：
6.1
作者：
Engle-Stone, R;Yeung, A;Glahn, R
通讯作者：
Glahn, R
DOI：
10.1024/0300-9831.75.6.371
发表时间：
2005-11-01
影响因子：
2.3
作者：
Fairweather-Tait, S;Lynch, S;Trinidad, TP
通讯作者：
Trinidad, TP
影响因子：
7.1
作者：
Rappaport, Aviva I.;Whitfield, Kyly C.;Green, Timothy J.
通讯作者：
Green, Timothy J.
影响因子：
8.8
作者：
Rodriguez-Ramiro I;Brearley CA;Bruggraber SF;Perfecto A;Shewry P;Fairweather-Tait S
通讯作者：
Fairweather-Tait S
影响因子：
5.9
作者：
Perfecto A;Elgy C;Valsami-Jones E;Sharp P;Hilty F;Fairweather-Tait S
通讯作者：
Fairweather-Tait S