Parallel recording of neurotransmitters release from chromaffin cells using a 10×10 CMOS IC potentiostat array with on-chip working electrodes.
Parallel recording of neurotransmitters release from chromaffin cells using a 10×10 CMOS IC potentiostat array with on-chip working electrodes.
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DOI：
10.1016/j.bios.2012.09.058
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发表时间：
2013-03-15
影响因子：
12.6
通讯作者：
Lindau M
中科院分区：
文献类型：
--
作者：
Kim BN;Herbst AD;Kim SJ;Minch BA;Lindau M
神经递质的释放受多种药物和分子调控。我们提出了一种用于神经递质释放的片上安培测量的高通量(100个电极)的有源的基于cmos的电化学生物传感器阵列。生物传感器阵列的高通量将加快所需的数据收集，以确定在不同条件下产生的变化的统计意义，从几周到几个小时。该生物传感器的设计和制造采用了CMOS集成电路(IC)技术和光刻工艺相结合的方法，将铂工作电极集成到芯片上。我们演示了集成高增益恒电位器和具有最小死区读出时间的输出时分多路复用的电极阵列的工作原理。芯片上的工作电极是通过共形沉积铂和剥离光刻来形成图案的。保形沉积方法在测量期间保护底层电子电路不与覆盖电极阵列的电解液接触。通过同时测量100个电极对多巴胺注入的响应电流，揭示了多巴胺在生物传感器阵列表面扩散的时间过程，验证了该生物传感器的有效性。该生物传感器同时成功地记录了多个活的嗜铬细胞的神经递质释放。该生物传感器能够分辨报告从单个囊泡分泌物中释放的小而快速的安培尖峰。我们预计，该设备将通过对神经元和内分泌细胞的药物和分子操作来加速表征神经递质分泌的调节。
Neurotransmitter release is modulated by many drugs and molecular manipulations. We present an active CMOS-based electrochemical biosensor array with high throughput capability (100 electrodes) for on-chip amperometric measurement of neurotransmitter release. The high-throughput of the biosensor array will accelerate the data collection needed to determine statistical significance of changes produced under varying conditions, from several weeks to a few hours. The biosensor is designed and fabricated using a combination of CMOS integrated circuit (IC) technology and a photolithography process to incorporate platinum working electrodes on-chip. We demonstrate the operation of an electrode array with integrated high-gain potentiostats and output time-division multiplexing with minimum dead time for readout. The on-chip working electrodes are patterned by conformal deposition of Pt and lift-off photolithography. The conformal deposition method protects the underlying electronic circuits from contact with the electrolyte that covers the electrode array during measurement. The biosensor was validated by simultaneous measurement of amperometric currents from 100 electrodes in response to dopamine injection, which revealed the time course of dopamine diffusion along the surface of the biosensor array. The biosensor simultaneously recorded neurotransmitter release successfully from multiple individual living chromaffin cells. The biosensor was capable of resolving small and fast amperometric spikes reporting release from individual vesicle secretions. We anticipate that this device will accelerate the characterization of the modulation of neurotransmitter secretion from neuronal and endocrine cells by pharmacological and molecular manipulations of the cells.
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影响因子：
12.6
作者：
Heer, F.;Hafizovic, S.;Hierlemann, A.
通讯作者：
Hierlemann, A.
影响因子：
21.3
作者：
Gong, Liang-Wei;Alvarez de Toledo, Guillermo;Lindau, Manfred
通讯作者：
Lindau, Manfred
影响因子：
16.2
作者：
Bruns, D;Riedel, D;Jahn, R
通讯作者：
Jahn, R
影响因子：
64.8
作者：
CHOW, RH;VONRUDEN, L;NEHER, E
通讯作者：
NEHER, E
影响因子：
64.8
作者：
BRUNS, D;JAHN, R
通讯作者：
JAHN, R