DNA Antenna Tile-Associated Deoxyribozyme Sensor with Improved Sensitivity.
DNA Antenna Tile-Associated Deoxyribozyme Sensor with Improved Sensitivity.
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DOI：
10.1002/cbic.201600438
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发表时间：
2016-11-03
期刊：
影响因子：
3.2
通讯作者：
Kolpashchikov, Dmitry M.
中科院分区：
文献类型：
--
作者：
Cox, Amanda J.;Bengtson, Hillary N.;Gerasimova, Yulia V.;Rohde, Kyle H.;Kolpashchikov, Dmitry M.
关键词：
一些天然酶通过促进底物流向其活性位点来增加扩散限制反应的速率。受这种自然现象的启发，我们开发了一种有效的底物传递到脱氧核酶（DZ）催化传感器的策略。这导致其灵敏度提高3至4倍，检测限提高9倍。该方法可用于提高扩散限制酶的催化效率和提高酶基生物传感器的灵敏度。用于核酸荧光检测的脱氧核酶（DZ）传感器在附着于DNA瓦片时表现出提高的灵敏度和检测极限，DNA瓦片吸引并增加荧光报告底物的局部浓度。
Some natural enzymes increase the rate of diffusion-limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in 3- to 4-fold increase in its sensitivity and up to 9-fold improvement of the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion-limited enzymes and to improve sensitivity of enzyme-based biosensors. Deoxyribozyme (DZ) sensors for fluorescent detection of nucleic acids demonstrate improved sensitivities and limits of detection when attached to a DNA tile, which attracts and increases local concentration of the fluorogenic reporter substrate.
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影响因子：
3.2
作者：
Bengtson, Hillary N.;Kolpashchikov, Dmitry M.
通讯作者：
Kolpashchikov, Dmitry M.
影响因子：
3.2
作者：
Gerasimova, Yulia V.;Cornett, Evan;Kolpashchikov, Dmitry M.
通讯作者：
Kolpashchikov, Dmitry M.
影响因子：
12.6
作者：
Bone, Simon M.;Lima, Nicole E.;Todd, Alison V.
通讯作者：
Todd, Alison V.
DOI：
10.1089/108729002761381276
发表时间：
2002-10-01
期刊：
ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT
影响因子：
--
作者：
Dass, CR;Saravolac, EG;Sun, LQ
通讯作者：
Sun, LQ
影响因子：
4.4
作者：
Buchner, Alexander;Tostevin, Filipe;Gerland, Ulrich
通讯作者：
Gerland, Ulrich