Phytoplankton trigger the production of cryptic metabolites in the marine actinobacterium Salinispora tropica.
Phytoplankton trigger the production of cryptic metabolites in the marine actinobacterium Salinispora tropica.
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DOI：
10.1111/1751-7915.13722
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发表时间：
2021-01
影响因子：
5.7
通讯作者：
Christie-Oleza JA
中科院分区：
文献类型：
--
作者：
Chhun A;Sousoni D;Aguiló-Ferretjans MDM;Song L;Corre C;Christie-Oleza JA
热带盐孢菌与浮游植物的相互作用海洋光养生物释放的光合产物触发了热带链霉菌新型隐蔽代谢物的生物合成。热带链霉菌产生一种未知的抗菌分子，杀死浮游植物。 放线菌门的丝状菌是具有药用潜力的天然产物的重要来源。然而，从这些生物体中发现新分子受到阻碍，因为编码这些次级代谢物的大多数生物合成基因簇（BGC）是隐蔽的或沉默的，并且被称为孤儿BGC。虽然共培养已被证明是通过激活这些孤儿BGC的表达来释放许多微生物的生物合成潜力的有前途的方法，但它仍然是一种未开发的技术。例如，海洋放线菌Salinispora tropica产生有价值的化合物，如抗癌分子salinosporamide，但其一半的推定BGC仍然是孤儿。虽然以前的研究已经使用海洋异养生物在Salinispora中诱导孤儿BGC，但其与海洋光养生物的共培养还有待研究。热带链霉菌对一系列浮游植物的抗菌活性观察后，我们在这里报告，光合初级生产者释放的光合产物影响其生物合成能力与生产的隐蔽分子和孤儿BGC的激活。我们的工作，使用代谢组学和蛋白质组学相结合的方法，开创了利用光养生物作为一个有前途的战略，以加快发现新的天然产物从海洋放线菌。
Interaction of Salinispora tropica with phytoplankton. Marine phototrophs release photosynthate that triggers the biosynthesis of novel cryptic metabolites in S. tropica. S. tropica produces an unknown antimicrobial molecule that kills phytoplankton. Filamentous members of the phylum Actinobacteria are a remarkable source of natural products with pharmaceutical potential. The discovery of novel molecules from these organisms is, however, hindered because most of the biosynthetic gene clusters (BGCs) encoding these secondary metabolites are cryptic or silent and are referred to as orphan BGCs. While co‐culture has proven to be a promising approach to unlock the biosynthetic potential of many microorganisms by activating the expression of these orphan BGCs, it still remains an underexplored technique. The marine actinobacterium Salinispora tropica, for instance, produces valuable compounds such as the anti‐cancer molecule salinosporamide but half of its putative BGCs are still orphan. Although previous studies have used marine heterotrophs to induce orphan BGCs in Salinispora, its co‐culture with marine phototrophs has yet to be investigated. Following the observation of an antimicrobial activity against a range of phytoplankton by S. tropica, we here report that the photosynthate released by photosynthetic primary producers influences its biosynthetic capacities with production of cryptic molecules and the activation of orphan BGCs. Our work, using an approach combining metabolomics and proteomics, pioneers the use of phototrophs as a promising strategy to accelerate the discovery of novel natural products from marine actinobacteria.
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影响因子：
28.3
作者：
Christie-Oleza JA;Sousoni D;Lloyd M;Armengaud J;Scanlan DJ
通讯作者：
Scanlan DJ
DOI：
10.1073/pnas.1714381115
发表时间：
2017-12-26
影响因子：
11.1
作者：
Amos, Gregory C. A.;Awakawa, Takayoshi;Jensen, Paul R.
通讯作者：
Jensen, Paul R.
影响因子：
16.6
作者：
Feling, RH;Buchanan, GO;Fenical, W
通讯作者：
Fenical, W
DOI：
10.1038/ja.2009.114
发表时间：
2010-01
期刊：
The Journal of antibiotics
影响因子：
--
作者：
通讯作者：
--
影响因子：
64.8
作者：
Guidi L;Chaffron S;Bittner L;Eveillard D;Larhlimi A;Roux S;Darzi Y;Audic S;Berline L;Brum J;Coelho LP;Espinoza JCI;Malviya S;Sunagawa S;Dimier C;Kandels-Lewis S;Picheral M;Poulain J;Searson S;Tara Oceans coordinators;Stemmann L;Not F;Hingamp P;Speich S;Follows M;Karp-Boss L;Boss E;Ogata H;Pesant S;Weissenbach J;Wincker P;Acinas SG;Bork P;de Vargas C;Iudicone D;Sullivan MB;Raes J;Karsenti E;Bowler C;Gorsky G
通讯作者：
Gorsky G