Effects of Coptis extract combined with chemotherapeutic agents on ROS production, multidrug resistance, and cell growth in A549 human lung cancer cells.
Effects of Coptis extract combined with chemotherapeutic agents on ROS production, multidrug resistance, and cell growth in A549 human lung cancer cells.
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DOI：
10.1186/1749-8546-7-11
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发表时间：
2012-04-30
期刊：
影响因子：
4.9
通讯作者：
Kang JX
中科院分区：
文献类型：
--
作者：
He C;Rong R;Liu J;Wan J;Zhou K;Kang JX
非小细胞肺癌与多药耐药（MDR）蛋白的高表达和活性氧（ROS）的低产生有关。黄连提取物（Coptis extract，COP）是一种中草药，其主要成分小檗碱（berberine，BER）具有抗癌作用.本研究旨在探讨COP和BER联合化疗药物（包括氟尿嘧啶（5-FU）、喜树碱（CPT）和紫杉醇（TAX））对A549人非小细胞肺癌细胞增殖、ROS产生和MDR的影响。用不同剂量的COP和BER联合5-FU、CPT和TAX处理A549细胞。通过XTT（2，3-双-（2-甲氧基-4-硝基-5-磺基苯基）-2H-四唑鎓-5-甲酰苯胺）测定来测量细胞活力。通过测量细胞可渗透的2′，7 ′-二氯荧光素二乙酸酯向荧光二氯荧光素的氧化转化来测定细胞内ROS水平。罗丹明123滞留法检测A549细胞的多药耐药情况。COP和BER对A549细胞的生长均有明显的抑制作用，且呈剂量依赖性。COP或BER与化疗药物（5-FU、CPT和TAX）联合应用对A549细胞生长有较强的抑制作用。此外，COP和BER可增加A549细胞中ROS的产生，减少MDR。COP和BER作为非小细胞肺癌化疗的潜在辅助剂，可增加ROS的产生，减少MDR，增强化疗药物对A549细胞生长的抑制作用。
Non–small cell lung cancer is associated with high expression of multidrug resistance (MDR) proteins and low production of reactive oxygen species (ROS). Coptis extract (COP), a Chinese medicinal herb, and its major constituent, berberine (BER), have anticancer properties. This study aims to investigate the effects of COP and BER combined with chemotherapeutic agents, including fluorouracil (5-FU), camptothecin (CPT), and paclitaxel (TAX), on cell proliferation, ROS production, and MDR in A549 human non-small cell lung cancer cells. A549 cells were treated with different doses of COP and BER, combined with 5-FU, CPT, and TAX. Cell viability was measured by an XTT (2,3-bis-(2-methoxy-4- nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide) assay. Intracellular ROS levels were determined by measuring the oxidative conversion of cell permeable 2′,7′-dichlorofluorescein diacetate to fluorescent dichlorofluorescein. MDR of A549 cells was assessed by rhodamine 123 retention assay. Both COP and BER significantly inhibited A549 cell growth in a dose-dependent manner. Combinations of COP or BER with chemotherapeutic agents (5-FU, CPT, and TAX) exhibited a stronger inhibitory effect on A549 cell growth. In addition, COP and BER increased ROS production and reduced MDR in A549 cells. As potential adjuvants to chemotherapy for non–small cell lung cancer, COP and BER increase ROS production, reduce MDR, and enhance the inhibitory effects of chemotherapeutic agents on A549 cell growth.
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影响因子：
45.3
作者：
Giaccone, G;Herbst, RS;Johnson, DH
通讯作者：
Johnson, DH
影响因子：
3.7
作者：
Liu B;Wang G;Yang J;Pan X;Yang Z;Zang L
通讯作者：
Zang L
影响因子：
3.8
作者：
Lin, Tseng-Hsi;Kuo, Hsing-Chun;Chou, Fen-Pi;Lu, Fung-Jou
通讯作者：
Lu, Fung-Jou
影响因子：
7.2
作者：
Simon, HU;Haj-Yehia, A;Levi-Schaffer, F
通讯作者：
Levi-Schaffer, F
影响因子：
6.1
作者：
Fontaine, M;Elmquist, WF;Miller, DW
通讯作者：
Miller, DW