Translational control of gene function through optically regulated nucleic acids.
Translational control of gene function through optically regulated nucleic acids.
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DOI：
10.1039/d1cs00257k
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发表时间：
2021-11-29
影响因子：
46.2
通讯作者：
Deiters A
中科院分区：
文献类型：
--
作者：
Darrah KE;Deiters A
mRNA翻译成蛋白质是生物系统中最基本的过程之一。基因表达在空间和时间上都受到严格的调控，通常涉及复杂的信号传导或基因调控网络，这在胚胎发育中最为突出。因此，基因功能的研究需要与外部控制水平相匹配的工具。光是一种优秀的条件触发，因为它是微创的，可以很容易地调谐波长和振幅，并可以以优异的空间和时间分辨率应用。为此，修改已建立的基于多核苷酸的技术与光学控制元件，在光笼组和光开关的形式，使这些工具能够在细胞和体内模型中的mRNA翻译的动态调控途径导航。在这篇综述中，我们讨论了不同的光化学方法用于产生光敏核酸，激活和灭活基因的表达和功能在翻译水平。基因功能可以通过光响应核酸精确控制。
Translation of mRNA into protein is one of the most fundamental processes within biological systems. Gene expression is tightly regulated both in space and time, often involving complex signaling or gene regulatory networks, as most prominently observed in embryo development. Thus, studies of gene function require tools with a matching level of external control. Light is an excellent conditional trigger as it is minimally invasive, can be easily tuned in wavelength and amplitude, and can be applied with excellent spatial and temporal resolution. To this end, modification of established oligonucleotide-based technologies with optical control elements, in the form of photocaging groups and photoswitches, has rendered these tools capable of navigating the dynamic regulatory pathways of mRNA translation in cellular and in vivo models. In this review, we discuss the different optochemical approaches used to generate photoresponsive nucleic acids that activate and deactivate gene expression and function at the translational level. Gene function can be precisely controlled with light-responsive nucleic acids.
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影响因子：
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作者：
Connelly CM;Uprety R;Hemphill J;Deiters A
通讯作者：
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影响因子：
3.2
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