Multiomics Study Reveals Enterococcus and Subdoligranulum Are Beneficial to Necrotizing Enterocolitis.
Multiomics Study Reveals Enterococcus and Subdoligranulum Are Beneficial to Necrotizing Enterocolitis.
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多组学研究表明肠球菌和小颗粒有益于坏死性小肠结肠炎
DOI：
10.3389/fmicb.2021.752102
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wen J
中科院分区：
文献类型：
--
作者：
Lin H;Guo Q;Ran Y;Lin L;Chen P;He J;Chen Y;Wen J
坏死性小肠结肠炎（NEC）是低体重早产儿的一种危及生命的疾病。由于其脆弱的肠道微生物组和粪便微生物群移植（FMT）治疗肠道疾病的成功，我们旨在揭示FMT和/或磺胺酮治疗后的多组学变化。本研究采用2周龄新生家兔模拟NEC模型，分为健康对照组、NEC组、舒拉酮组、FTM组和FMT与舒拉酮联合组。我们评估了肠道病理和生存率，以确定每种治疗的益处，并进行了微生物组和转录组分析，以揭示微观水平的变化，这有助于了解NEC的发病机制和制定新的策略。我们发现NEC兔从FMT和磺胺酮联合治疗中获益更多。联合治疗逆转了许多被NEC失调的微生物，并显示出与健康对照最相似的转录谱。此外，FMT与舒拉酮联合使用可显著延长NEC家兔的存活时间。功能富集表明，代谢和病毒生命周期是NEC最显著的变化。FMT是NEC常用的治疗方法。同时，在NEC合并肠道感染的严重情况下，首选的治疗策略是第三代头孢菌素，其中舒拉酮应用广泛，效果显著。所以，我们用磺胺酮治疗有NEC的兔子。在本研究中，我们旨在探讨FMT和舒拉酮以及两者联合使用对NEC的不同影响。考虑到微生物组和转录组的结果，我们得出结论，肠球菌和亚doligranulum分别通过影响细菌噬菌体和丁酸盐的产生对NEC有益。
Necrotizing enterocolitis (NEC) is a life-threatening disease for premature infants with low body weight. Due to its fragile gut microbiome and successful treatment of fecal microbiota transplantation (FMT) for intestinal disease, we aimed to reveal the multiple-omics changes after FMT and/or sulperazone treatment. In this study, 2-week-old newborn rabbits were used to simulate the NEC model and grouped into healthy control, NEC, sulperazone treatment, FTM treatment, and FMT and sulperazone combination treatment. We evaluated the intestinal pathology and survival to define the benefit from each treatment and performed microbiome and transcriptome analysis to reveal the changes in microcosmic level, which could be helpful to understand the pathogenesis of NEC and develop new strategy. We found NEC rabbits benefit more from the combination of FMT and sulperazone treatment. Combination treatment reverses a lot of microorganisms dysregulated by NEC and showed the most similar transcript profiler with healthy control. Moreover, a combination of FMT and sulperazone significantly prolonged the survival of NEC rabbits. Function enrichment showed that metabolism and viral life cycle are the most significant changes in NEC. FMT is a common therapy method for NEC. Meanwhile, in the severe situation of NEC with intestinal infection, the first therapy strategy is preferred the third-generation cephalosporin, among which sulperazone is used widely and the effect is remarkable. So, we used sulperazone to treat the rabbits with the NEC. In this research, we aim to explore the different effects on NEC between FMT and sulperazone as well as the combination. Considering the microbiome and transcriptome result, we make a conclusion that the Enterococcus and Subdoligranulum benefits NEC by influencing the bacterial phages and butyrate production, respectively.
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影响因子：
4.4
作者：
Esber N;Mauras A;Delannoy J;Labellie C;Mayeur C;Caillaud MA;Kashima T;Souchaud L;Nicolis I;Kapel N;Waligora-Dupriet AJ
通讯作者：
Waligora-Dupriet AJ
影响因子：
5.9
作者：
Plaza-Díaz J;Fontana L;Gil A
通讯作者：
Gil A
早产儿坏死性小肠结肠炎和迟发性败血症相关的肠道菌群模式进展：中国新生儿重症监护病房的一项前瞻性研究
影响因子：
2.7
作者：
Liu, Jiayi;Li, Yuqing;Hong, Li
通讯作者：
Hong, Li
影响因子：
1.1
作者：
Bozeman, Andrew P.;Dassinger, Melvin S.;Smith, Samuel D.
通讯作者：
Smith, Samuel D.
DOI：
10.1126/science.aao3290
发表时间：
2018-01-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Matson V;Fessler J;Bao R;Chongsuwat T;Zha Y;Alegre ML;Luke JJ;Gajewski TF
通讯作者：
Gajewski TF