Seven-year surveillance of emm types of pediatric Group A streptococcal pharyngitis isolates in Western Greece.
Seven-year surveillance of emm types of pediatric Group A streptococcal pharyngitis isolates in Western Greece.
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DOI：
10.1371/journal.pone.0071558
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
van der Linden M
中科院分区：
文献类型：
--
作者：
Syrogiannopoulos GA;Grivea IN;Al-Lahham A;Panagiotou M;Tsantouli AG;Michoula Ralf René Reinert AN;van der Linden M
一种实验性的26价M蛋白A组链球菌（GAS）疫苗已进入临床研究。不同地区、不同时段的咽部GAS emm型监测，可提高对GAS咽炎流行病学的认识。此外，这些监测与GAS侵袭性疾病的数据相结合，可以在多价型特异性疫苗的配制中发挥重要作用。在7年期间（1999 - 2005年），2408 GAS分离株从希腊西部连续儿童咽炎中回收。总的大环内酯类耐药率为22.8%。沿着研究期间，我们注意到耐药率显着下降的趋势，最低耐药率出现在2002年（15.3%）、2003年（15%）和2004年（16.7%）。每年随机抽取548株大环内酯类耐药菌株中的338株（61.7%）和205株（11%）大环内酯类敏感菌株进行分子分析，包括emm分型。543个分型菌株有28个不同的emm型。大环内酯类药物耐药与emm4、emm22和emm77之间存在统计学显著相关性，而emm1、emm3、emm6、emm12、emm87和emm89与大环内酯类药物敏感性相关。emm4、emm28和emm77的年变化幅度较大。最常见的大环内酯类耐药GAS是携带单独或与mef组合的Emm77（A）、携带mef的Emm4（A）、携带Emm28（B）、携带mef的Emm75（A）、携带mef的Emm12（A）和携带Emm22（A）。我们估计82.8%的分离株属于新型26价M蛋白疫苗中包含的emm型。疫苗覆盖率主要由非疫苗emm4分离株频率的增加决定。在大环内酯类药物敏感和耐药的GAS分离株中，数量有限的EMM类型占主导地位。我们观察到了季节性波动，这对emm4，emm28和emm77来说很重要。如果新型26价M蛋白GAS疫苗被引入实践，这种类型的数据可以作为基线信息。
An experimental 26-valent M protein Group A streptococcal (GAS) vaccine has entered clinical studies. Pharyngeal GAS emm type surveillances in different areas and time-periods enhance the understanding of the epidemiology of GAS pharyngitis. Moreover, these surveillances, combined with the data on GAS invasive disease, can play a significant role in the formulation of multivalent type-specific vaccines. During a 7-year period (1999–2005), 2408 GAS isolates were recovered from consecutive children with pharyngitis in Western Greece. The overall macrolide resistance rate was 22.8%. Along the study period we noted a tendency towards significantly decreased rates of resistance, with the lowest rates occurring in 2002 (15.3%), 2003 (15%) and 2004 (16.7%). A random sample of isolates from each year, 338 (61.7%) of the 548 macrolide-resistant and 205 (11%) of the macrolide-susceptible, underwent molecular analysis, including emm typing. The 543 typed isolates had 28 different emm types. A statistically significant association was found between macrolide resistance and emm4, emm22 and emm77, whereas emm1, emm3, emm6, emm12, emm87 and emm89 were associated with macrolide susceptibility. A significant yearly fluctuation was observed in emm4, emm28 and emm77. The most common macrolide-resistant GAS were emm77 isolates harboring erm(A), either alone or in combination with mef(A), emm4 carrying mef(A), emm28 possessing erm(B), emm75 carrying mef(A), emm12 harboring mef(A) and emm22 carrying erm(A). We estimated that 82.8% of the isolates belonged to emm types included in the novel 26-valent M protein vaccine. The vaccine coverage rate was determined mainly by the increased frequency of nonvaccine emm4 isolates. A limited number of emm types dominated among macrolide-susceptible and macrolide-resistant GAS isolates. We observed seasonal fluctuations, which were significant for emm4, emm28 and emm77. This type of data can serve as baseline information if the novel 26-valent M protein GAS vaccine is introduced into practice.
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DOI：
10.1084/jem.161.3.623
发表时间：
1985-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Jones KF;Manjula BN;Johnston KH;Hollingshead SK;Scott JR;Fischetti VA
通讯作者：
Fischetti VA
影响因子：
11.8
作者：
O'Loughlin, Rosalyn E.;Roberson, Angela;Van Beneden, Chris
通讯作者：
Van Beneden, Chris
影响因子：
11.8
作者：
Darenberg, Jessica;Luca-Harari, Bogdan;Normark, Birgitta Henriques
通讯作者：
Normark, Birgitta Henriques
影响因子：
4.9
作者：
Giovanetti, E;Montanari, MP;Varaldo, PE
通讯作者：
Varaldo, PE
影响因子：
3.6
作者：
Jaggi, Preeti;Dale, James B.;Shulman, Stanford T.
通讯作者：
Shulman, Stanford T.