Dynamics of interdomain and intermolecular interactions in mammalian metallothioneins.
Dynamics of interdomain and intermolecular interactions in mammalian metallothioneins.
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哺乳动物金属硫蛋白结构域间和分子间相互作用的动力学。
DOI：
10.1016/s0162-0134(01)00379-8
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发表时间：
2002
影响因子：
3.9
通讯作者：
Armitage,IanM
中科院分区：
文献类型：
--
作者：
Zangger,Klaus;Armitage,IanM
The structures of mammalian metallothioneins (MTs), as solved by X-ray crystallography and NMR spectroscopy, all show seven divalent metals bound in two separate domains. The marked differences in metal-mobilities found for the two domains has led to the proposal for a dual role for the two MT metal domains. The tight metal binding in the C-terminal α-domain supposedly constitutes the basis for the detoxification of excess heavy metals, while the more labile metals in the N-terminal β-domain function in the homeostasis of the essential elements zinc and copper. In this overview, we compare the two types of dimers found for MTs and their influence on metal-mobilities. In the presence of excess metal, the N-terminal domain is responsible for the formation of metal-bridged dimers while under aerobic conditions, a specific intermolecular disulfide is formed between the C-terminal domains. Both forms of dimers not only involve different domains for their intermolecular protein interactions, they also exhibit radical differences in the reactive properties of their respective cluster bound metal ions. Since the metal exchange within each domain is also influenced by interdomain interactions, the relative orientation of the domains is also most likely important for MT functions. Thus far, the relative orientation of the two domains could only be obtained from the crystal structure. Here, we present evidence for increased mobility in the linker region as the reason for the lack of interdomain constraints in the solution NMR studies of mammalian MTs.
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拟胆碱激动剂和β-肾上腺素受体兴奋剂之间的拮抗作用。
影响因子：
5
作者：
J. Offermeier;F. G. van den Brink
通讯作者：
F. G. van den Brink
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DOI：
10.1152/jappl.1982.52.4.1084
发表时间：
1982
期刊：
Journal of applied physiology: respiratory, environmental and exercise physiology
影响因子：
--
作者：
Murlas,C;Nadel,JA;Roberts,JM
通讯作者：
Roberts,JM
从分离的犬气管细胞制备激素敏感的气道平滑肌腺苷酸环化酶。
DOI：
--
发表时间：
1981
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
G. A. Rinard;A. Jensen
通讯作者：
A. Jensen
DOI：
10.1073/pnas.81.21.6899
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BARON, CB;CUNNINGHAM, M;COBURN, RF
通讯作者：
COBURN, RF
异丙肾上腺素对犬气道收缩的差异抑制作用。
DOI：
10.1152/jappl.1984.57.3.801
发表时间：
1984
期刊：
Journal of applied physiology: respiratory, environmental and exercise physiology
影响因子：
--
作者：
Russell,JA
通讯作者：
Russell,JA