NERD1 is required for primexine formation and plasma membrane undulation during microsporogenesis in Arabidopsis thaliana
NERD1 is required for primexine formation and plasma membrane undulation during microsporogenesis in Arabidopsis thaliana
复制标题
拟南芥小孢子发生过程中 NERD1 是 Primexine 形成和质膜波动所必需的
DOI：
10.1007/s42994-020-00022-1
复制
发表时间：
2020-06
期刊：
影响因子：
--
通讯作者：
Wanqi Liang
中科院分区：
文献类型：
--
作者：
Dawei Xu;Palash Ch;ra Mondol;Sumie Ishiguro;Jianxin Shi;Dabing Zhang;Wanqi Liang
初外层形成和质膜波动是许多被子植物花粉壁形成的关键步骤。然而，这些过程的分子机制在很大程度上尚不清楚。据报道，在拟南芥中，根矮化新增强子 1 (NERD1)（一种跨膜蛋白）在植物发育中发挥多效性作用，包括雄性生育力控制；然而，NERD1 破坏如何影响男性生殖尚不清楚。在这里，我们发现 nerd1 突变体的雄性不育归因于花粉壁形成早期步骤的缺陷。我们发现nerd1-2没有底外层形成和小孢子质膜波动，胼胝质沉积有缺陷。因此，孢粉素前体无法在小孢子表面沉积和组装，而是在花药室和绒毡层细胞中积累，最终导致小孢子败育。 NERD1定位于高尔基体，在营养器官和生殖器官中表达，其中在生殖组织中表达最高，包括绒毡层、雄性性母细胞、四分体和成熟花粉粒。我们的结果表明 NERD1 对于底壁沉积和小孢子质膜波动是必需的，因此对于孢子花粉组装和花粉外壁形成至关重要。
The primexine formation and plasma membrane undulation are the crucial steps of pollen wall for-mation in many angiosperms. However, the molecular mechanism underlining these processes is largely unknown. In Arabidopsis, NEW ENHANCER OF ROOT DWARFISM1 (NERD1), a transmembrane protein, was reported to play pleiotropic roles in plant development including male fertility control; while, how NERD1 disruption impacts male reproduction is yet unclear. Here, we revealed that the male sterility of nerd1 mutants is attributed to defects in early steps of pollen wall formation. We found that nerd1-2 is void of primexine formation and microspore plasma membrane undulation, defective in callose deposition. Consequently, sporopollenin precursors are unable to deposit and assemble on the microspore surface, but instead accumulated in the anther locule and tapetal cells, and ultimately leading to microspore abortion. NERD1 is localized in the Golgi and is expressed in both vegetative and reproductive organs, with the highest expression in reproductive tissues, including the tapetum, male meiocytes, tetrads and mature pollen grains. Our results suggest that NERD1 is required for the primexine deposition and microspore plasma membrane undulation, thus essential for sporopollenin assembly and pollen exine formation.
登录
查看更多内容
拟南芥花粉壁图案
影响因子：
18.9
作者：
Xu, Te;Zhang, Cheng;Zhou, Que;Yang, Zhong-Nan
通讯作者：
Yang, Zhong-Nan
影响因子：
4.5
作者：
Jing Yuan;S. Kessler
通讯作者：
Jing Yuan;S. Kessler
影响因子：
7.2
作者：
Wilson, ZA;Morroll, SM;Tighe, PJ
通讯作者：
Tighe, PJ
DEX1 是一种质膜定位蛋白，通过影响拟南芥中 CalS5 的表达而在小孢子发育中发挥作用
影响因子：
--
作者：
Ma LiJuan;Yang ZhongNan;Zhang Sen
通讯作者：
Zhang Sen
DOI：
10.1002/j.1460-2075.1987.tb02730.x
发表时间：
1987-12
期刊：
The EMBO Journal
影响因子：
--
作者：
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者：
R. Jefferson;T. Kavanagh;M. Bevan