Appearance, fate and utilization of abnormal porcine embryos produced by in vitro maturation and fertilization.
Appearance, fate and utilization of abnormal porcine embryos produced by in vitro maturation and fertilization.
复制标题
体外成熟和受精产生的异常猪胚胎的外观、命运和利用。
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
T. Nagai
中科院分区：
文献类型：
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作者：
K. Kikuchi;T. Somfai;M. Nakai;T. Nagai
体外生产（IVP）包括体外成熟（IVM）和受精（IVF），现在是获得活仔猪的重要技术。然而，有效生产有活力的猪胚胎仍然存在两个显著障碍：（1）多精受精和（2）未成熟阶段卵母细胞的受精。这些现象与产生具有异常倍性（多倍性）的胚胎有关。为了避免这些问题，仔细选择成熟的卵母细胞进行IVF，并定期监测正常和异常受精（多精受精和/或缺乏雄性原核形成）是非常重要的。然而，在我们最近的研究中，我们已经证实，一些异常倍性的卵母细胞在多精受精后可以发育成具有潜在正常发育能力的二倍体胚胎。这种受精的多倍体卵母细胞在胚胎发育过程中发育到正常状态的机制仍然没有很好的理解。试图澄清这一机制将有望揭示不仅对IVP而且对其他技术非常有用的数据，例如使用IVM卵母细胞生产转基因或克隆动物，包括其他物种，也用于人类生殖操作。本文就猪体外成熟卵母细胞的正常性和体外成熟胚胎的倍性进行了综述，并提出了解决上述问题的可行途径。
In vitro production (IVP) including in vitro maturation (IVM) and fertilization (IVF) is now an important technology for obtaining live piglets. However, there are still two significant obstacles to the efficient production of viable porcine embryos: (1) polyspermy and (2) fertilization of oocytes arrested at the immature stage. These phenomena relate to production of embryos with abnormal ploidy (polyploidy). To avoid these problems, careful selection of mature oocytes for IVF, and regular monitoring of normal and abnormal fertilization (polyspermy and/or lack of male pronucleus formation) are very important. In our recent studies, however, we have confirmed that some oocytes with abnormal ploidy after polyspermy can develop into diploid embryos with potentially normal developmental ability. The mechanism by which such fertilized polyploid oocytes develop to a normal state during embryo development is still not well understood. Attempts to clarify this mechanism would hopefully reveal data that are very useful for not only IVP but also other technologies such as the production of transgenic or cloned animals using IVM oocytes, including other species, also for human reproductive manipulation. In this review, we focus on studies of normality of IVM oocytes and ploidy of IVP embryos, and try to suggest practical ways of solving the problems mentioned above in pigs.
影响因子：
2.7
作者：
EPPIG, JJ;SCHULTZ, RM;CHESNEL, F
通讯作者：
CHESNEL, F