Analysis of Biological Interactions by Affinity Chromatography: Clinical and Pharmaceutical Applications.
Analysis of Biological Interactions by Affinity Chromatography: Clinical and Pharmaceutical Applications.
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DOI：
10.1373/clinchem.2016.262253
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发表时间：
2017-06
影响因子：
9.3
通讯作者：
Hage DS
中科院分区：
文献类型：
--
作者：
Hage DS
体内生化和化学物质之间的相互作用在许多临床过程中都很重要。亲和层析和高性能亲和层析（HPAC）是两种可用于研究这些相互作用的方法，其中一柱含有固定化的生物相关结合剂。本文综述了亲和色谱和HPAC中用于表征生物相互作用强度或速率、参与这一过程的位点的数量和类型以及同一结合剂的多个溶质之间的相互作用的各种方法。研究了这些方法的许多应用，重点是最近的发展和高性能亲和方法。这些应用包括将这些技术用于生物相互作用的基础研究、药物的高通量筛选、修饰蛋白的工作、个性化医疗的工具以及研究一种常见结合剂的药物-药物竞争。广泛的格式和检测方法可以与亲和色谱和HPAC一起用于检查生物相互作用，使这些工具对各种临床和制药应用具有吸引力。小规模色谱柱的未来发展方向，以及这些方法与其他技术（如质谱法或其他分离方法）的耦合，应该继续增加这些方法在涉及临床或药物样品的工作中使用的灵活性和便利性。
The interactions between biochemical and chemical agents in the body are important in many clinical processes. Affinity chromatography and high-performance affinity chromatography (HPAC), in which a column contains an immobilized biologically-related binding agent, are two methods that can be used to study these interactions. This review looks at various approaches that can be used in affinity chromatography and HPAC to characterize the strength or rate of a biological interaction, the number and types of sites that are involved in this process, and the interactions between multiple solutes for the same binding agent. A number of applications for these methods are examined, with an emphasis on recent developments and high-performance affinity methods. These applications include the use of these techniques for fundamental studies of biological interactions, high-throughput screening of drugs, work with modified proteins, tools for personalized medicine, and studies of drug-drug competition for a common binding agent. The wide range of formats and detection methods that can be used with affinity chromatography and HPAC for examining biological interactions makes these tools attractive for various clinical and pharmaceutical applications. Future directions in the development of small-scale columns and the coupling of these methods with other techniques, such as mass spectrometry or other separation methods, should continue to increase the flexibility and ease with which these approaches can be used in work involving clinical or pharmaceutical samples.
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DOI：
10.1016/j.cca.2013.07.013
发表时间：
2013-10-21
期刊：
Clinica chimica acta; international journal of clinical chemistry
影响因子：
--
作者：
Anguizola J;Matsuda R;Barnaby OS;Hoy KS;Wa C;DeBolt E;Koke M;Hage DS
通讯作者：
Hage DS
DOI：
10.1016/0021-9673(92)80159-r
发表时间：
1992-09-18
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
FITOS, I;VISY, J;HERMANSSON, J
通讯作者：
HERMANSSON, J
影响因子：
2.9
作者：
Chen S;Sobansky MR;Hage DS
通讯作者：
Hage DS
影响因子：
4.3
作者：
Anguizola J;Bi C;Koke M;Jackson A;Hage DS
通讯作者：
Hage DS
影响因子：
4.1
作者：
Calleri, E.;Fracchiolla, G.;Temporini, C.
通讯作者：
Temporini, C.