PPR Protein BFA2 Is Essential for the Accumulation of the atpH/F Transcript in Chloroplasts
PPR Protein BFA2 Is Essential for the Accumulation of the atpH/F Transcript in Chloroplasts
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PPR 蛋白 BFA2 对于叶绿体中 atpH/F 转录物的积累至关重要
DOI：
10.3389/fpls.2019.00446
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发表时间：
2019-04
影响因子：
5.6
通讯作者：
Peng Lianwei
中科院分区：
文献类型：
--
作者：
Zhang Lin;Zhou Wen;Che Liping;Rochaix Jean David;Lu Congming;Li Wenjing;Peng Lianwei
叶绿体ATP合成酶是一种复杂而迷人的纳米生物，由核基因组和质体基因组共同编码的9个亚基组成。由于其不均匀的亚基化学计量，ATP合酶的生物合成和质体编码的ATP合酶基因的表达需要参与转录、转录后和翻译步骤的核编码因子的协助。在这项研究中，我们报告了一个P-类五肽重复（PPR）蛋白BFA 2（ATP合成酶所需的生物发生因子2），这是必不可少的积累的双顺反子atpH/F转录在拟南芥叶绿体。BFA 2的功能缺失突变导致叶绿体ATP合酶特异性减少3/4以上，这可能是由于缺乏双顺反子atpH/F转录物。BFA 2蛋白含有22个推测的PPR基序，并且仅定位于叶绿体中。生物信息学和电泳迁移率变动分析（EMSA）表明BFA 2以序列特异性方式结合atpF-atpA基因间区的共有序列。然而，atpA的翻译起始在bfa 2突变体中不受影响。因此，我们认为叶绿体PPR蛋白BFA 2主要通过与atpF-atpA基因间区的共有序列结合来作为屏障，以防止atpH/F转录物被核糖核酸外切酶降解。
As a fascinating and complicated nanomotor, chloroplast ATP synthase comprises nine subunits encoded by both the nuclear and plastid genomes. Because of its uneven subunit stoichiometry, biogenesis of ATP synthase and expression of plastid-encoded ATP synthase genes requires assistance by nucleus-encoded factors involved in transcriptional, post-transcriptional, and translational steps. In this study, we report a P-class pentatricopeptide repeat (PPR) protein BFA2 (Biogenesis Factor required for ATP synthase 2) that is essential for accumulation of the dicistronic atpH/F transcript in Arabidopsis chloroplasts. A loss-of-function mutation in BFA2 results in a specific reduction of more than 3/4 of chloroplast ATP synthase, which is likely due to the absence of dicistronic atpH/F transcript. BFA2 protein contains 22 putative PPR motifs and exclusively localizes in the chloroplast. Bioinformatics and Electrophoretic Mobility Shift Assays (EMSA) analysis showed that BFA2 binds to the consensus sequence of the atpF-atpA intergenic region in a sequence-specific manner. However, translation initiation of the atpA was not affected in the bfa2 mutant. Thus, we propose that the chloroplast PPR protein BFA2 mainly acts as barrier to prevent the atpH/F transcript degradation by exoribonucleases by binding to the consensus sequence of the atpF-atpA intergenic region.
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影响因子：
3
作者：
Wendl MC
通讯作者：
Wendl MC
DOI：
10.1073/pnas.1612460114
发表时间：
2017-02
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Wen-jiao Zhou;Qingtao Lu;Qingwei Li;Lei Wang;Shunhua Ding;A. Zhang;Xiaogang Wen;Lixin Zhang
通讯作者：
Wen-jiao Zhou;Qingtao Lu;Qingwei Li;Lei Wang;Shunhua Ding;A. Zhang;Xiaogang Wen;Lixin Zhang
影响因子：
11.6
作者：
McCormac, DJ;Barkan, A
通讯作者：
Barkan, A
影响因子：
3.7
作者：
Fristedt R;Martins NF;Strenkert D;Clarke CA;Suchoszek M;Thiele W;Schöttler MA;Merchant SS
通讯作者：
Merchant SS
影响因子：
7.4
作者：
Lin Zhang;Zhikun Duan;Jiao Zhang;Lianwei Peng
通讯作者：
Lin Zhang;Zhikun Duan;Jiao Zhang;Lianwei Peng