SARS-CoV-2 Neutralizing Responses in Various Populations, at the Time of SARS-CoV-2 Variant Virus Emergence: Evaluation of Two Surrogate Neutralization Assays in Front of Whole Virus Neutralization Test.
SARS-CoV-2 Neutralizing Responses in Various Populations, at the Time of SARS-CoV-2 Variant Virus Emergence: Evaluation of Two Surrogate Neutralization Assays in Front of Whole Virus Neutralization Test.
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DOI：
10.3390/life12122064
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发表时间：
2022-12-09
期刊：
影响因子：
3.2
通讯作者：
Gozlan, Joel
中科院分区：
文献类型：
--
作者：
Marot, Stephane;Fofana, Djeneba Bocar;Flandre, Philippe;Malet, Isabelle;Zafilaza, Karen;Leducq, Valentin;Vivien, Diane;Mrabet, Sarah;Poignon, Corentin;Calvez, Vincent;Morand-Joubert, Laurence;Marcelin, Anne-Genevieve;Gozlan, Joel
SARS-CoV-2中和抗体反应是感染和/或接种疫苗后有效保护的最佳指标，但其评估需要使用感染性病毒进行繁琐的基于细胞的实验。我们分析了105例有各种SARS-CoV-2感染和/或疫苗接种史的患者，使用病毒中和试验（VNT）对B.1，Alpha，Beta和Omicron变体的中和反应，并将结果与基于抗体介导的ACE 2-RBD相互作用阻断的两种替代试验（Lateral Flow Boditech和ELISA Genscript）进行了比较。在接受一剂疫苗的COVID-19康复患者中观察到最强的反应。接受2剂mRNA疫苗的初治患者也显示出对B.1、α和β变体的高中和滴度，但仅34.3%显示出对Omicron变体的中和活性。另一方面，未感染的患者与一半的疫苗接种计划显示了对所有分离株的弱和不稳定的活动。未接种疫苗的COVID-19患者在康复后12个月内保持对B.1和Alpha的中和活性，但对Beta和Omicron的活性下降。两种替代检测均显示与VNT具有良好的相关性。然而，有必要调整阳性临界值，特别是对于最耐药的Beta和Omicron变体。我们验证了两种简单可靠的替代中和试验，这可能有利于取代基于细胞的方法，允许更大规模的功能分析。
The SARS-CoV-2 neutralizing antibodies response is the best indicator of effective protection after infection and/or vaccination, but its evaluation requires tedious cell-based experiments using an infectious virus. We analyzed, in 105 patients with various histories of SARS-CoV-2 infection and/or vaccination, the neutralizing response using a virus neutralization test (VNT) against B.1, Alpha, Beta and Omicron variants, and compared the results with two surrogate assays based on antibody-mediated blockage of the ACE2-RBD interaction (Lateral Flow Boditech and ELISA Genscript). The strongest response was observed for recovered COVID-19 patients receiving one vaccine dose. Naïve patients receiving 2 doses of mRNA vaccine also demonstrate high neutralization titers against B.1, Alpha and Beta variants, but only 34.3% displayed a neutralization activity against the Omicron variant. On the other hand, non-infected patients with half vaccination schedules displayed a weak and inconstant activity against all isolates. Non-vaccinated COVID-19 patients kept a neutralizing activity against B.1 and Alpha up to 12 months after recovery but a decreased activity against Beta and Omicron. Both surrogate assays displayed a good correlation with the VNT. However, an adaptation of the cut-off positivity was necessary, especially for the most resistant Beta and Omicron variants. We validated two simple and reliable surrogate neutralization assays, which may favorably replace cell-based methods, allowing functional analysis on a larger scale.
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影响因子：
82.9
作者：
Lucas C;Klein J;Sundaram ME;Liu F;Wong P;Silva J;Mao T;Oh JE;Mohanty S;Huang J;Tokuyama M;Lu P;Venkataraman A;Park A;Israelow B;Vogels CBF;Muenker MC;Chang CH;Casanovas-Massana A;Moore AJ;Zell J;Fournier JB;Yale IMPACT Research Team;Wyllie AL;Campbell M;Lee AI;Chun HJ;Grubaugh ND;Schulz WL;Farhadian S;Dela Cruz C;Ring AM;Shaw AC;Wisnewski AV;Yildirim I;Ko AI;Omer SB;Iwasaki A
通讯作者：
Iwasaki A
DOI：
10.1016/j.jcv.2021.105024
发表时间：
2021-12
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Lake DF;Roeder AJ;Kaleta E;Jasbi P;Pfeffer K;Koelbela C;Periasamy S;Kuzmina N;Bukreyev A;Grys TE;Wu L;Mills JR;McAulay K;Gonzalez-Moa M;Seit-Nebi A;Svarovsky S
通讯作者：
Svarovsky S
影响因子：
8.8
作者：
Hirabidian, Mickael;Bocket, Laurence;Demaret, Julie;Vuotto, Fanny;Rabat, Anthony;Faure, Karine;Labalette, Myriam;Hober, Didier;Lefevre, Guillaume;Alidjinou, Enagnon Kazali
通讯作者：
Alidjinou, Enagnon Kazali
DOI：
10.1056/nejmoa2109072
发表时间：
2021-10-14
期刊：
The New England journal of medicine
影响因子：
--
作者：
Bergwerk M;Gonen T;Lustig Y;Amit S;Lipsitch M;Cohen C;Mandelboim M;Levin EG;Rubin C;Indenbaum V;Tal I;Zavitan M;Zuckerman N;Bar-Chaim A;Kreiss Y;Regev-Yochay G
通讯作者：
Regev-Yochay G
影响因子：
11.8
作者：
Marot, Stephane;Malet, Isabelle;Jary, Aude
通讯作者：
Jary, Aude