Evaluation of a rapid semiquantitative lateral flow assay for the prediction of serum neutralizing activity against SARS-CoV-2 variants.
Evaluation of a rapid semiquantitative lateral flow assay for the prediction of serum neutralizing activity against SARS-CoV-2 variants.
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DOI：
10.1016/j.jcv.2022.105268
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发表时间：
2022-10
影响因子：
8.8
通讯作者：
Alidjinou, Enagnon Kazali
中科院分区：
文献类型：
--
作者：
Hirabidian, Mickael;Bocket, Laurence;Demaret, Julie;Vuotto, Fanny;Rabat, Anthony;Faure, Karine;Labalette, Myriam;Hober, Didier;Lefevre, Guillaume;Alidjinou, Enagnon Kazali
针对SARS-CoV-2的中和抗体（NAb）已被证明与预防感染相关。简单的工具，如侧流测定（LFA），可以准确地测量NAb可能是有用的，在未来监测抗SARS-CoV-2免疫。我们评估了ichroma™ COVID-19 nAb检测的性能，这是一种快速半定量LFA，用于预测SARS-CoV-2变体的血清中和活性。从COVID-19康复患者和接种疫苗的个体中采集血清样本。将ichroma测定的结果提供为抑制率，并与抗SARS-CoV-2 IgG水平和针对α、δ和Omicron变体的NAb进行比较。本研究共纳入了90份来自未接种疫苗患者的血清和209份来自疫苗队列的血清。在感染后样本中，发现色素瘤抑制率与IgG水平（ρ = 0.83）和抗α NAbs水平（ρ = 0.78）相关。在疫苗队列中，还观察到变色瘤抑制率与IgG水平（ρ = 0.84）以及针对Alpha（ρ = 0.62）、Delta（ρ = 0.88）和Omicron（ρ = 0.74）的NAb之间具有良好的相关性。77.2%、90.8%和99.6%的ichroma抑制率分别准确预测了对α、δ和Omicron变体的中和。ichroma™ COVID-19 nAb检测试剂盒（具有适当的变异截止值）可用于监测抗SARS-CoV-2免疫接种，并可提供快速预测保护，尤其是在NAb水平显著的个体中。
Neutralizing antibodies (NAbs) against SARS-CoV-2 have been shown to correlate with protection against infection. Simple tools such as lateral flow assays (LFA) that can accurately measure NAbs may be useful for monitoring anti-SARS-CoV-2 immunity in the future. We assessed the performance of the ichroma™ COVID-19 nAb test, a rapid semiquantitative LFA, for the prediction of serum neutralizing activity against SARS-CoV-2 variants. Serum samples were collected from COVID-19 recovered patients and vaccinated individuals. The result of the ichroma assay was provided as inhibition rate, and was compared to anti-SARS-CoV-2 IgG levels, and NAbs against Alpha, Delta and Omicron variants. A total of 90 sera from recovered unvaccinated patients and 209 sera from the vaccine cohort were included in this study. In post-infection samples, the ichroma inhbition rate was found to be correlated with IgG levels (ρ = 0.83), and with anti-Alpha NAbs levels (ρ = 0.78). In the vaccine cohort, a good correlation was also observed between the ichroma inhibition rate and IgG levels (ρ = 0.84), as well as NAbs against Alpha (ρ = 0.62), Delta (ρ = 0.88) and Omicron (ρ = 0.74). An ichroma inhbition rate of 77.2%, 90.8% and 99.6% accurately predicted neutralization against Alpha, Delta and Omicron variants respectively. The ichroma™ COVID-19 nAb assay, with appropriate variant cut-offs, can be useful for the monitoring of anti-SARS-CoV-2 immunization and may provide a rapid prediction of protection, especially in individuals with significant levels of NAbs.
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影响因子：
9.4
作者：
Saker K;Escuret V;Pitiot V;Massardier-Pilonchéry A;Paul S;Mokdad B;Langlois-Jacques C;Rabilloud M;Goncalves D;Fabien N;Guibert N;Fassier JB;Bal A;Trouillet-Assant S;Trabaud MA
通讯作者：
Trabaud MA
影响因子：
5.8
作者：
Sander I;Kespohl S;Zahradnik E;Göcke P;Hosbach I;Herrmann BL;Brüning T;Raulf M
通讯作者：
Raulf M
DOI：
10.1016/j.jcv.2021.105024
发表时间：
2021-12
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Lake DF;Roeder AJ;Kaleta E;Jasbi P;Pfeffer K;Koelbela C;Periasamy S;Kuzmina N;Bukreyev A;Grys TE;Wu L;Mills JR;McAulay K;Gonzalez-Moa M;Seit-Nebi A;Svarovsky S
通讯作者：
Svarovsky S
DOI：
10.1038/s41577-020-00471-1
发表时间：
2020-12
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Khoury DS;Wheatley AK;Ramuta MD;Reynaldi A;Cromer D;Subbarao K;O'Connor DH;Kent SJ;Davenport MP
通讯作者：
Davenport MP
影响因子：
15.3
作者：
Schmidt, Fabian;Weisblum, Yiska;Bieniasz, Paul D.
通讯作者：
Bieniasz, Paul D.