Characteristics of the cofactor requirements for the superoxide-generating NADPH oxidase of human polymorphonuclear leukocytes.
Characteristics of the cofactor requirements for the superoxide-generating NADPH oxidase of human polymorphonuclear leukocytes.
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人多形核白细胞产生超氧化物的 NADPH 氧化酶的辅因子需求特征。
DOI：
10.1021/bi00509a010
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Tauber,AI
中科院分区：
文献类型：
--
作者：
Light,DR;Walsh,C;O'Callaghan,AM;Goetzl,EJ;Tauber,AI
David R.Light，ChristopherWalsh，Angela M.O‘Callaghan，Edward J.Goetzl和Alfred I.Tauber*摘要：利用亚细胞微粒和溶解的制剂，研究了减少烟酰胺腺嘌呤二核苷酸磷酸(NADPH)和其他辅因子要求的在人多形核(PMN)白细胞中诱发的超氧化物(OF·)生成活性。颗粒制剂的产生率与刺激物无关，但优化酵母多糖的NADPH氧化与产生量之比约为佛波醇醋酸酯刺激物的2倍。相反，在离子或非离子洗涤剂中增溶的制剂，无论在何种刺激条件下，NADPH氧化与产生·的比率是相似的。在亚细胞颗粒中用(4/？)-[2*H]NADPH取代(4/？)-[1H]NADPH时，对Fmax的同位素效应比在溶解制剂中更大，因此NADPH的氧化限速程度取决于酶的物理状态和纯度。·的生成率和自失活率
David R. Light, ChristopherWalsh, Angela M. O’Callaghan, Edward J. Goetzl, and Alfred I. Tauber* abstract: Reduced nicotinamide adenine dinucleotide phosphate (NADPH) and other cofactor requirements of the superoxide (Of·) generating activity evoked in human polymorphonuclear (PMN) leukocytes were characterized by utilizing subcellular particulate and solubilized preparations. Particulate preparations exhibited equalrates of Of· gener-ation irrespective of the stimulus, but the ratio of NADPH oxidation to Of· generation was approximately 2-fold lower with opsonized zymosan than with phorbol myristate acetate as the stimulus. In contrast, the ratio of NADPH oxidation to Of· generation was similar for preparations solubilized in ionic or nonionic detergents regardless of the stimulus. A greater isotope effect on Fmax was observed when (4/?)-[2*** H] NADPH was substituted for (4/?)-[1H] NADPH in sub-cellular particles than in solubilized preparations, so that the extent to which the oxidation of NADPH is rate limiting is dependent on the physical state and purity of the oxidase. Both the rates of Of· generation and of autoinactivation of the
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人类中性粒细胞的颗粒 O-2 形成系统产生氧化自由基的证据。
影响因子：
20.3
作者：
A. Tauber;T. Gabig;B. Babior
通讯作者：
B. Babior
DOI：
10.1016/0005-2744(73)90180-0
发表时间：
1973-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
WEVER, R;VANGELDE.BF
通讯作者：
VANGELDE.BF
DOI：
10.1016/0006-291x(69)90288-5
发表时间：
1969-01-01
影响因子：
3.1
作者：
BALLOU, D;PALMER, G;MASSEY, V
通讯作者：
MASSEY, V
影响因子：
2.9
作者：
H. Misra;I. Fridovich
通讯作者：
I. Fridovich
细胞色素 b5 与磷脂酰胆碱囊泡的结合。
DOI：
--
发表时间：
1973
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
M. Sullivan;P. W. Holloway
通讯作者：
P. W. Holloway