Early forms of microtubule-associated protein are strongly expressed in cortical dysplasia
Early forms of microtubule-associated protein are strongly expressed in cortical dysplasia
复制标题
早期形式的微管相关蛋白在皮质发育不良中强烈表达
作者：
H. Yamanouchi;V. Jay;H. Otsubo;M. Kaga;L. Becker;S. Takashima
摘要我们报道了17例难治性癫痫患儿手术切除皮质发育不良中早期微管相关蛋白（MAPs）的表达增强。大的神经元，这代表了皮质发育不良的特征性细胞功能之一，表现出强烈的免疫反应性的MAP1B，以及低分子量亚型的MAP2（MAP2C）。MAP1B反义核酸探针原位杂交显示，大的神经元中的杂交信号强度明显增加，而在正常外观的皮质中的神经元和发育不良皮质中的大多数正常大小的神经元中有微弱的信号。由于MAP2c和MAP1B是MAPs的早期形式，它们在发育中的大脑中大量表达，在成人中下调，并且被认为参与神经元的生长和可塑性，因此我们的研究结果表明，在皮质发育不良中，神经元过程的结构重塑被激活。
Abstract We report the enhanced expression of early forms of microtubule-associated proteins (MAPs) in cortical dysplasia in surgical resections from 17 children with intractable epilepsy. Large neurons, which represent one of the characteristic cellular features of cortical dysplasia, showed strong immunoreactivity for MAP1B, as well as the low-molecular-weight isoform of MAP2 (MAP2c). Insitu hybridization with MAP1B antisense riboprobe showed markedly increased hybridization signal intensities in the large neurons, whereas neurons in the normal-appearing cortex and most of the normal-sized neurons in the dysplastic cortex had faint signals. Because MAP2c and MAP1B are early forms of MAPs, which are abundantly expressed in the developing brain and down-regulated in the adult, and are thought to be involved in neuronal outgrowth and plasticity, our results suggest that the structural remodeling of neuronal processes is activated in cortical dysplasia.
DOI：
10.1073/pnas.93.3.1270
发表时间：
1996-02-06
影响因子：
11.1
作者：
Edelmann, W;Zervas, M;Kucherlapati, R
通讯作者：
Kucherlapati, R
微管相关蛋白 (MAP) 在成年和胚胎小鼠视网膜外植体中的分布：胚胎图谱 MAP5/1B 在再生成年视网膜轴突中的存在。
影响因子：
2.7
作者：
Bates,CA;Trinh,N;Meyer,RL
通讯作者：
Meyer,RL