Determination of LMF binding site on a HSA-PPIX complex in the presence of human holo transferrin from the viewpoint of drug loading on proteins.
Determination of LMF binding site on a HSA-PPIX complex in the presence of human holo transferrin from the viewpoint of drug loading on proteins.
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DOI：
10.1371/journal.pone.0084045
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chamani J
中科院分区：
文献类型：
--
作者：
Sattar Z;Saberi MR;Chamani J
全转铁蛋白 (TF) 和人血清白蛋白与原卟啉 IX (HSA-PPIX) 的天然复合物是两种可以相互作用的血清载体蛋白。这种相互作用可能会改变它们的结合位点。在本研究中，荧光光谱以及 zeta 电位和分子建模技术已用于比较复合物 (HSA-PPIX)-LMF 和 [(HSA-PPIX)-TF]-LMF。 (HSA-PPIX)-LMF和[(HSA-PPIX)-TF]-LMF的Ka1、Ka2值分别为1.1×105 M−1、9.7×106 M−1和2.0×104 M−1、1.8×105 M−1，n1、n2值分别为1.19、1.53和1.17， 1.65。 (HSA-PPIX)-LMF 的色氨酸发射扫描的二阶导数在 310 nm 处显示出一个负带，而对于 [(HSA-PPIX)-TF]-LMF 系统，我们在 316 nm 处观察到一个负带，表明色氨酸周围的极性增加。使用三维荧光光谱分析了TF对(HSA-PPIX)-TF构象的影响。相图表明HSA和TF上第二个结合位点的存在是由于中间结构的存在。 Zeta电位分析表明TF的存在增加了HSA-PPIX系统的正电荷。位点标记实验表明，在存在 TF 的情况下，LMF 与 HSA-PPIX 的结合位点从 Sudlow 位点 IIA 变为 Sudlow 位点 IIIB。此外，分子模型研究表明HSA中的子结构域IIIB是在(HSA-PPIX)-TF复合物上形成LMF结合位点的候选位置。
Holo transferrin (TF) and the natural complex of human serum albumin and protoporphyrin IX (HSA-PPIX) are two serum carrier proteins that can interact with each other. Such an interaction may alter their binding sites. In this study, fluorescence spectroscopy, as well as zeta potential and molecular modeling techniques, have been used to compare the complexes (HSA-PPIX)-LMF and [(HSA-PPIX)-TF]-LMF. The Ka1, Ka2, values of (HSA-PPIX)-LMF and [(HSA-PPIX)-TF]-LMF were 1.1×105 M−1, 9.7×106 M−1, and 2.0×104 M−1, 1.8×105 M−1, respectively, and the n1, n2 values were respectively 1.19, 1.53 and 1.17, 1.65. The second derivative of the Trp emission scan of (HSA-PPIX)-LMF exhibited one negative band at 310 nm, whereas for the [(HSA-PPIX)-TF]-LMF system, we observed one negative band at 316 nm indicating an increase in polarity around Trp. The effect of TF on the conformation of (HSA-PPIX)-TF was analyzed using three-dimensional fluorescence spectroscopy. The phase diagram indicated that the presence of a second binding site on HSA and TF was due to the existence of intermediate structures. Zeta potential analysis showed that the presence of TF increased the positive charges of the HSA-PPIX system. Site marker experiments revealed that the binding site of LMF to HSA-PPIX changed from Sudlow's site IIA to Sudlow's site IIIB in the presence of TF. Moreover, molecular modeling studies suggested the sub-domain IIIB in HSA as the candidate place for the formation of the binding site of LMF on the (HSA-PPIX)-TF complex.
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影响因子：
3.3
作者：
De Luca, G;Romeo, A;Scolaro, LM
通讯作者：
Scolaro, LM
DOI：
10.1016/j.jphotochem.2007.05.018
发表时间：
2007-12-15
影响因子：
4.3
作者：
Nanda, Ranjan Kumar;Sarkar, Nilmoni;Banerjee, Rintu
通讯作者：
Banerjee, Rintu
使用三维激发发射矩阵荧光光谱表征预臭氧化浸没式膜生物反应器中的有机膜污染物
影响因子：
12.8
作者：
Liu, Ting;Chen, Zhong-lin;You, Shi-jie
通讯作者：
You, Shi-jie
DOI：
10.1111/j.1432-1033.1983.tb07540.x
发表时间：
1983-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
DUNACH, M;SABES, M;PADROS, E
通讯作者：
PADROS, E
影响因子：
13.8
作者：
PADROS, E;DUNACH, M;MANOSA, J
通讯作者：
MANOSA, J