Enhanced Interleukin-6 and Interleukin-8 Synthesis in Term and Preterm Infants
Enhanced Interleukin-6 and Interleukin-8 Synthesis in Term and Preterm Infants
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增强足月儿和早产儿中白细胞介素 6 和白细胞介素 8 的合成
DOI：
10.1203/00006450-200203000-00009
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发表时间：
2002
影响因子：
3.6
通讯作者：
P. Bucsky
中科院分区：
文献类型：
--
作者：
C. Schultz;C. Rott;P. Temming;P. Schlenke;J. Möller;P. Bucsky
越来越多的证据表明，新生儿败血症相关并发症是由促炎细胞因子介导的。先前已经证明，升高的IL-6和IL-8水平可以预测早产儿的脑损伤和慢性肺部疾病。然而，目前的观点是新生儿产生促炎细胞因子的能力降低。为了澄清这个问题，我们通过流式细胞术直接在单细胞水平上分析了足月儿和早产儿的炎症反应。在规定的条件下，对50名健康成人和119名新生儿（包括45名足月儿、63名早产儿（胎龄26.1-36.7周）和11名确诊感染的早产儿（24.6-29.9周））的单核细胞进行内毒素激发试验。我们的研究结果通过证明足月儿和早产儿比成人显示出更高百分比的IL-6和IL-8阳性细胞来挑战现有的不成熟炎症反应的观点。在与地塞米松预孵育后，所有组中的IFN-γ阳性细胞数量均减少，但与成人相比，足月和>32周的早产儿中IL-8阳性细胞数量仍较高。这些观察结果表明，在足月儿和早产儿中，不仅发育良好，而且炎症反应增强。考虑到IL-6和IL-8的几种有害作用，我们的数据可能对炎症引发的新生儿疾病的病理生理学产生重大影响。
There is growing evidence that sepsis-related complications in neonates are crucially mediated by the action of proinflammatory cytokines. It has previously been demonstrated that elevated IL-6 and IL-8 levels can predict brain damage and chronic lung disease in preterm infants. However, it is the current view that neonates have a reduced capability to produce proinflammatory cytokines. To clarify this issue, we analyzed the inflammatory response in term and preterm infants directly at the single cell level by flow cytometry. Endotoxin challenge was performed under defined conditions on monocytes obtained from 50 healthy adults and 119 neonates, which consist of 45 term infants, 63 preterm infants (26.1–36.7 wk of gestational age), and 11 preterm infants with proven infection (24.6–29.9 wk). Our results challenge the existing view of an immature inflammatory response by demonstrating that term infants and preterm infants display a higher percentage of IL-6– and IL-8–positive cells than adults. After preincubation with dexamethasone the number of cytokine-positive cells decreased in all groups, but the number of IL-8–positive cells remained higher in term and preterm infants >32 wk compared with adults. These observations demonstrate not only a well-developed but also an enhanced inflammatory response in term and preterm infants. Under consideration of several detrimental effects of IL-6 and IL-8, our data may have major implications on the pathophysiology of inflammatory-triggered neonatal diseases.
B 族链球菌从人类单核细胞中引发白三烯 B4 和白细胞介素 8：新生儿的反应减弱。
DOI：
10.1093/infdis/172.2.420
发表时间：
1995
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Rowen,JL;Smith,CW;Edwards,MS
通讯作者：
Edwards,MS