Single basic amino acid substitutions at position 302 or 320 in the V3 domain of HIV type 1 are not sufficient to alter the antiviral activity of dextran sulfate and heparin.
Single basic amino acid substitutions at position 302 or 320 in the V3 domain of HIV type 1 are not sufficient to alter the antiviral activity of dextran sulfate and heparin.
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1 型 HIV V3 结构域中第 302 或 320 位的单个碱性氨基酸取代不足以改变硫酸葡聚糖和肝素的抗病毒活性。
DOI：
10.1089/aid.1995.11.571
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Gurney,ME
中科院分区：
文献类型：
--
作者：
Okada,T;Gurney,ME
The third variable domain (V3 domain) of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120 contains a substantial number of positively charged amino acid residues. We previously demonstrated that mutation of basic amino acid residues at position 303, 306, 309, 313, and 325 in the V3 domain of HIV-1 strain NL4-3 resulted in a dramatic elimination of both virus infectivity and syncytium-inducing ability. Mutations of arginine at position 302 to serine (R302S) or lysine at position 320 to glutamine (K320Q) had variable effects on infectivity for a panel of T cell lines tested. These mutations are located on opposite sides of the Gly-Pro-Gly-Arg-Ala sequence in the center of the V3 domain. The R302S and K320Q mutations allowed us to determine if these basic residues are important for virus neutralization by polyanionic compounds. Dextran sulfate and heparin inhibited the cytopathogenicities of both mutants for MT-4 cells, although their 50% antiviral effective doses were slightly higher than those required to achieve complete protection against wild-type HIV-1NL4-3replication. This result emphasizes that the basic amino acids of Arg302and Lys320are not essential for the inhibitory effect of dextran sulfate and heparin on HIV-1 infection.
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影响因子：
4.4
作者：
Seth Lederman;Roy M. Gulick;Leonard Chess
通讯作者：
Seth Lederman;Roy M. Gulick;Leonard Chess
G.S.利维：科学。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.9
作者：
BABA, M;SCHOLS, D;GOROG, S
通讯作者：
GOROG, S
影响因子：
56.9
作者：
KOWALSKI, M;POTZ, J;SODROSKI, J
通讯作者：
SODROSKI, J
影响因子：
5.4
作者：
DEJONG, JJ;GOUDSMIT, J;DERONDE, A
通讯作者：
DERONDE, A