Changes in Hepatic Insulin-Like Growth Factor-I and β-Actin mRNA Levels in Chickens during
Changes in Hepatic Insulin-Like Growth Factor-I and β-Actin mRNA Levels in Chickens during
复制标题
鸡体内胰岛素样生长因子-I和β-肌动蛋白mRNA水平的变化
DOI：
10.2508/chikusan.68.752
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
J. Okumura
中科院分区：
文献类型：
--
作者：
K. Kita;K. Hangsanet;J. Okumura
我们检测了鸡在孵化后的早期生长阶段肝脏胰岛素样生长因子- i （IGF-I）和β-肌动蛋白mRNA水平的变化。在0、1、2、4、6和8周龄时，采用核糖核酸酶保护法测定肝脏IGF-I和β-肌动蛋白mRNA水平。肝脏IGF-I mRNA水平在0 ~ 1wk时显著升高，此后在8wk时有升高的趋势，但不显著。肝β-肌动蛋白mRNA水平在整个实验过程中保持不变。IGF-I mRNA/β-actin mRNA比值在0 ~ 6周龄显著升高，随后呈下降趋势，但不显著。这些结果表明，在鸡孵化后的早期生长阶段，肝脏igf - 1基因的表达迅速增加。
We examined changes in hepatic insulin-like growth factor-I (IGF-I) and β-actin mRNA levels in chickens during early stages of growth after hatching. At 0, 1, 2, 4, 6 and 8 wk of age, hepatic IGF-I and β-actin mRNA levels were measured by using a ribonuclease protection assay. The IGF-I mRNA level in the liver significantly increased from 0 to 1wk of age, and thereafter the level tended to increase toward 8wk of age, but not significantly. The level of hepatic β-actin mRNA was maintained throughout the experiment. The IGF-I mRNA/β-actin mRNA ratio significantly increased form 0 to 6wk of age, and then tended to decrease but not significantly. These results indicate that hepatic IGF-I gene expression in chickens rapidly increases at early stages of growth after hatching.
影响因子：
4.8
作者：
K. Kikuchi;F. Buonomo;Y. Kajimoto;P. Rotwein
通讯作者：
K. Kikuchi;F. Buonomo;Y. Kajimoto;P. Rotwein