Effect of intestinal tapeworms on the gut microbiota of the common carp, Cyprinus carpio
Effect of intestinal tapeworms on the gut microbiota of the common carp, Cyprinus carpio
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肠道绦虫对鲤鱼肠道菌群的影响
DOI：
10.1186/s13071-019-3510-z
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发表时间：
2019-05-22
影响因子：
3.2
通讯作者：
Li, Wen X.
中科院分区：
文献类型：
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作者：
Fu, Pei P.;Xiong, Fan;Li, Wen X.
寄生原生动物，蠕虫，改变肠道微生物在哺乳动物，但很少有人知道肠道绦虫对肠道微生物在鱼类的影响。本研究以日本卡氏绦虫（Khawia japonensis）和细颈溶囊绦虫（Acetolytocestus tenuicollis）感染鲤鱼（Cyprinus carpio）后肠为研究对象，对鲤鱼后肠微生物区系的组成和多样性进行了研究。肠道含有由变形菌门、梭菌门和Tenericutes组成的核心微生物群。两种绦虫感染对微生物多样性和丰富度没有显著影响，但在属水平上改变了微生物组成。PCoA分析表明，感染和未感染鲤鱼的微生物群落不能相互区分。然而，Mantel测试表明，K。japonensis的丰度与微生物组成显著相关（P = 0.015），而A.细颈则无（P = 0.954）。根据Pearson相关分析，K. japonensis与以下肠道微生物群分类群表现出极显著（P 0.001）正相关性：Epulopiscium、U114、拟杆菌、梭菌和消化链球菌科（0.8 r 0.9）;与肠杆菌科、微球菌科、Rummelibacillus、Lysinibacillusboronitolerans、韦荣氏球菌科、草酸杆菌科、气单胞菌科（阴性）、Marinibacillus和Chitinilyticum（0.4 r 0.7）显著（P 0.05）相关。这些结果表明，肠道菌群的组成受到K。日本血吸虫感染此外，致病菌（劳森菌属和邻单胞菌属）的比例增加也与K。日本血吸虫感染，这可能因此增加疾病的可能性。
Parasitic protozoans, helminths, alter the gut microbiota in mammals, yet little is known about the influence of intestinal cestodes on gut microbiota in fish. In the present study, the composition and diversity of the hindgut microbiota were determined in the intestine of common carp (Cyprinus carpio) infected with two tapeworm species, Khawia japonensis and Atractolytocestus tenuicollis. The intestine contained a core microbiota composed of Proteobacteria, Fusobacteria and Tenericutes. Infection with the two cestode species had no significant effect on the microbial diversity and richness, but it altered the microbial composition at the genus level. PCoA analysis indicated that microbial communities in the infected and uninfected common carp could not be distinguished from each other. However, a Mantel test indicated that the abundance of K. japonensis was significantly correlated with the microbial composition (P = 0.015), while the abundance of A. tenuicollis was not (P = 0.954). According to Pearsonʼs correlation analysis, the abundance of K. japonensis exhibited an extremely significant (P < 0.001) positive correlation with the following gut microbiota taxa: Epulopiscium, U114, Bacteroides, Clostridium and Peptostreptococcaceae (0.8< r < 0.9); and a significant (P < 0.05) correlation with Enterobacteriaceae, Micrococcaceae, Rummeliibacillus, Lysinibacillusboronitolerans, Veillonellaceae, Oxalobacteraceae, Aeromonadaceae (negative), Marinibacillus and Chitinilyticum (0.4< r < 0.7). These results suggest that the composition of gut microbiota was somewhat affected by the K. japonensis infection. Additionally, increased ratios of pathogenic bacteria (Lawsonia and Plesiomonas) were also associated with the K. japonensis infection, which may therefore increase the likelihood of disease.