Analysis of proteome adaptation reveals a key role of the bacterial envelope in starvation survival.
Analysis of proteome adaptation reveals a key role of the bacterial envelope in starvation survival.
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DOI：
10.15252/msb.202211160
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发表时间：
2022-12
影响因子：
9.9
通讯作者：
中科院分区：
文献类型：
--
作者：
细菌在进入稳定期时重组其生理机能。这种重组的哪一部分提高了饥饿存活率是一个难题，因为生理学上的变化包括蛋白质组、包膜和细胞代谢的全面重组。在这项工作中，我们使用了快速生长和长期生存之间的几种权衡，对2,000多种大肠杆菌蛋白质进行了统计评分，以确定其与死亡率的全球相关性。综合排名使我们能够缩小与生存呈正相关的蛋白质组，并验证蛋白质子集的因果作用。值得注意的是，我们发现重要的生存基因与细胞包膜有关，即，由于维持大肠杆菌的被膜完整性在饥饿期间起着至关重要的作用，因此，在饥饿期间，被膜完整性的维持在周质和外膜中起着至关重要的作用。我们的研究结果揭示了外膜的一个新的保护特征，这增加了越来越多的证据，即外膜不仅是阻止非生物物质到达细胞质的屏障，而且对细菌的增殖和生存至关重要。大肠杆菌在各种生长条件下的蛋白质组学分析表明，生长和饥饿之间的权衡可以通过细菌将资源投入细胞包膜以降低其渗透性来解释，这以牺牲生长为代价提高了它们的寿命。
Bacteria reorganize their physiology upon entry to stationary phase. What part of this reorganization improves starvation survival is a difficult question because the change in physiology includes a global reorganization of the proteome, envelope, and metabolism of the cell. In this work, we used several trade‐offs between fast growth and long survival to statistically score over 2,000 Escherichia coli proteins for their global correlation with death rate. The combined ranking allowed us to narrow down the set of proteins that positively correlate with survival and validate the causal role of a subset of proteins. Remarkably, we found that important survival genes are related to the cell envelope, i.e., periplasm and outer membrane, because the maintenance of envelope integrity of E. coli plays a crucial role during starvation. Our results uncover a new protective feature of the outer membrane that adds to the growing evidence that the outer membrane is not only a barrier that prevents abiotic substances from reaching the cytoplasm but also essential for bacterial proliferation and survival. Proteomic analysis of Escherichia coli under various growth conditions reveals that a trade‐off between growth and starvation can be explained by bacteria investing resources into the cell envelope to reduce its permeability, which improves their lifespan at the expense of growth.
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影响因子：
3.6
作者：
Fontaine F;Stewart EJ;Lindner AB;Taddei F
通讯作者：
Taddei F
影响因子：
3.2
作者：
FARR, SB;TOUATI, D;KOGOMA, T
通讯作者：
KOGOMA, T
影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
4.8
作者：
Dukan, S;Nyström, T
通讯作者：
Nyström, T
影响因子：
7
作者：
Ammar, Constantin;Gruber, Markus;Zimmer, Ralf
通讯作者：
Zimmer, Ralf