A Large Family of Enzymes Responsible for the Modular Architecture of Nematode Pheromones.
A Large Family of Enzymes Responsible for the Modular Architecture of Nematode Pheromones.
复制标题
DOI：
10.1021/jacs.0c04223
复制
发表时间：
2020-08-12
影响因子：
15
通讯作者：
Butcher RA
中科院分区：
文献类型：
--
作者：
Faghih N;Bhar S;Zhou Y;Dar AR;Mai K;Bailey LS;Basso KB;Butcher RA
秀丽隐杆线虫产生一个广泛的信息素家族，称为蛔虫苷，其被来自初级代谢的各种基团修饰。这些修饰对于蛔虫苷在发育和各种行为中的各种活动是必不可少的，包括吸引，聚集，回避和觅食。这些不同的基团被添加到蛔虫苷中的机制知之甚少。在这里，我们确定了一个家庭的30多个酶，这是同源的哺乳动物羧酸酯水解酶（CES）的酶，并表明，这些酶的数量是负责的选择性添加特定的修改的蛔虫。通过稳定的同位素饲养实验，我们证明了体内活性的CES样酶，并提供直接的证据表明，酰基辅酶A合成酶ACS-7，这是以前牵连在某些修饰的附件，以阿罗汉果苷在C。相反，elegans通过β-氧化激活某些Ascarosides的侧链以缩短。我们的数据提供了一个关键的组合逻辑，产生不同的修改ascarosides，这将大大促进探索这些信息素在蠕虫的特定生物学功能。
The nematode Caenorhabditis elegans produces a broad family of pheromones, known as the ascarosides, that are modified with a variety of groups derived from primary metabolism. These modifications are essential for the diverse activities of the ascarosides in development and various behaviors, including attraction, aggregation, avoidance, and foraging. The mechanism by which these different groups are added to the ascarosides is poorly understood. Here, we identify a family of over thirty enzymes, which are homologous to mammalian carboxylic ester hydrolase (CES) enzymes, and show that a number of these enzymes are responsible for the selective addition of specific modifications to the ascarosides. Through stable isotope feeding experiments, we demonstrate the in vivo activity of the CES-like enzymes and provide direct evidence that the acyl-CoA synthetase ACS-7, which was previously implicated in the attachment of certain modifications to the ascarosides in C. elegans, instead activates the side chain of certain ascarosides for shortening through β-oxidation. Our data provide a key to the combinatorial logic that gives rise to different modified ascarosides, which should greatly facilitate the exploration of the specific biological functions of these pheromones in the worm.
登录
查看更多内容
影响因子：
8.6
作者：
Falcke JM;Bose N;Artyukhin AB;Rödelsperger C;Markov GV;Yim JJ;Grimm D;Claassen MH;Panda O;Baccile JA;Zhang YK;Le HH;Jolic D;Schroeder FC;Sommer RJ
通讯作者：
Sommer RJ
比较代谢组学揭示了丙烷剂的生物发生，这是秀丽隐杆线虫中小分子信号的模块化库。
影响因子：
15
作者：
von Reuss, Stephan H.;Bose, Neelanjan;Srinivasan, Jagan;Yim, Joshua J.;Judkins, Joshua C.;Sternberg, Paul W.;Schroeder, Frank C.
通讯作者：
Schroeder, Frank C.
影响因子：
21.1
作者：
Lian J;Nelson R;Lehner R
通讯作者：
Lehner R
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
15
作者：
Artyukhin AB;Zhang YK;Akagi AE;Panda O;Sternberg PW;Schroeder FC
通讯作者：
Schroeder FC