TINCR inhibits the proliferation and invasion of laryngeal squamous cell carcinoma by regulating miR-210/BTG2.
TINCR inhibits the proliferation and invasion of laryngeal squamous cell carcinoma by regulating miR-210/BTG2.
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TINCR通过调节miR-210/BTG2抑制喉鳞状细胞癌的增殖和侵袭。
DOI：
10.1186/s12885-021-08513-0
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发表时间：
2021-06-29
期刊：
影响因子：
3.8
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
He G;Pang R;Han J;Jia J;Ding Z;Bi W;Yu J;Chen L;Zhang J;Sun Y
终末分化诱导的 ncRNA (TINCR) 在表皮分化中发挥重要作用，并参与各种癌症的发展。采用qPCR检测TINCR在喉鳞状细胞癌（LSCC）组织和细胞系中的表达水平。生物信息网站预测了TINCR的潜在靶点。通过qPCR检测miR-210和BTG2基因的表达，通过western blot评估BTG2和Ki-67的蛋白水平。采用CCK-8法、划痕试验、Transwell小室评价LSCC细胞的增殖、侵袭、转移能力。通过生物信息学软件和荧光素酶报告基因分析研究了 TINCR、miR-210 和 BTG2 之间的关系。通过裸鼠的存活率和肿瘤生长来评估 TINCR 的体内功能。我们采用qRT-PCR检测喉鳞状细胞癌（LSCC）组织和细胞中TINCR的表达，发现癌组织中的表达量显着低于癌旁组织。此外，TINCR 高表达的患者预后较好。观察到 TINCR 过表达可抑制 LSCC 细胞的增殖和侵袭。 TINCR通过吸附miR-210发挥抗增殖和侵袭作用，显着促进喉鳞状细胞的增殖和侵袭。 miR-210 的过度表达被确定可以逆转 TINCR 的肿瘤抑制作用。 BTG2（抗增殖因子2）被确定为miR-210的靶基因，BTG2过表达抑制LSCC细胞的增殖和侵袭。 BTG2敲低解除了TINCR对LSCC增殖和侵袭的抑制作用。最后，与对照小鼠相比，TINCR 上调减缓了裸鼠异种移植肿瘤的生长，并显着提高了存活率。本研究结果提示TINCR通过调节miR-210/BTG2通路抑制LSCC的增殖和侵袭，参与细胞周期调控，可能成为LSCC治疗的靶点。在线版本包含可在 10.1186/s12885-021-08513-0 获取的补充材料。
Terminal differentiation-induced ncRNA (TINCR) plays an essential role in epidermal differentiation and is involved in the development of various cancers. qPCR was used to detect the expression level of TINCR in tissues and cell lines of laryngeal squamous cell carcinoma (LSCC). The potential targets of TINCR were predicted by the bioinformation website. The expression of miR-210 and BTG2 genes were detected by qPCR, and the protein levels of BTG2 and Ki-67 were evaluated by western blot. CCK-8 assay, scratch test, and transwell chamber were used to evaluate the proliferation, invasion, and metastasis ability of LSCC cells. The relationships among TINCR, miR-210, and BTG2 were investigated by bioinformatics software and luciferase reporter assay. The in vivo function of TINCR was accessed on survival rate and tumor growth in nude mice. We used qRT-PCR to detect the expression of TINCR in laryngeal squamous cell carcinoma (LSCC) tissues and cells and found significantly lower levels in cancer tissues compared with adjacent tissues. Additionally, patients with high TINCR expression had a better prognosis. TINCR overexpression was observed to inhibit the proliferation and invasion of LSCC cells. TINCR was shown to exert its antiproliferation and invasion effects by adsorbing miR-210, which significantly promoted the proliferation and invasion of laryngeal squamous cells. Overexpression of miR-210 was determined to reverse the tumour-suppressive effects of TINCR. BTG2 (anti-proliferation factor 2) was identified as the target gene of miR-210, and BTG2 overexpression inhibited the proliferation and invasion of LSCC cells. BTG2 knockdown relieved the inhibitory effects of TINCR on the proliferation and invasion of LSCC. Finally, TINCR upregulation slowed xenograft tumour growth in nude mice and significantly increased survival compared with control mice. The results of this study suggest that TINCR inhibits the proliferation and invasion of LSCC by regulating the miR-210/BTG2 pathway, participates in cell cycle regulation, and may become a target for the treatment of LSCC. The online version contains supplementary material available at 10.1186/s12885-021-08513-0.
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影响因子：
4.6
作者：
Chen Z;Liu Y;He A;Li J;Chen M;Zhan Y;Lin J;Zhuang C;Liu L;Zhao G;Huang W;Cai Z
通讯作者：
Cai Z
结合长非编码 RNA 表达谱和临床因素的列线图可预测膀胱癌患者的生存
影响因子：
5.2
作者：
Wang, Yifan;Du, Lutao;Wang, Chuanxin
通讯作者：
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影响因子：
3.4
作者：
Xu G;Yang H;Liu M;Niu J;Chen W;Tan X;Sun L
通讯作者：
Sun L
影响因子：
2.7
作者：
Liu, Yuehui;Ye, Fan
通讯作者：
Ye, Fan
长的非编码RNA TINCR充当竞争性内源性RNA，可通过在胃癌中启动miR-375来调节PDK1表达。
影响因子：
4
作者：
Chen Z;Liu H;Yang H;Gao Y;Zhang G;Hu J
通讯作者：
Hu J