Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center.
Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center.
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DOI：
10.1002/dvg.22013
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发表时间：
2012-03
期刊：
影响因子：
1.5
通讯作者：
Horb, Marko E.
中科院分区：
文献类型：
--
作者：
Pearl, Esther J.;Grainger, Robert M.;Guille, Matthew;Horb, Marko E.
非洲爪蟾是生物医学研究中必不可少的脊椎动物模型系统，为许多学科的重要发现做出了贡献，包括细胞生物学，分子生物学，生理学，发育生物学和神经生物学。然而，与其他模型系统不同，直到最近才建立了爪蟾的中央储存库/库存中心。与已经建立库存中心的小鼠、斑马鱼和苍蝇群落类似，爪蟾研究人员需要维持和分发数量迅速增长的近交、突变和转基因青蛙品系，以及沿着DNA和蛋白质资源，各个实验室都在努力有效地完成这一任务。在过去的五年中，两个资源中心成立，以满足这一需求：欧洲非洲爪蟾资源中心（EXRC）在英国的朴茨茅斯大学，和国家非洲爪蟾资源（NXR）在海洋生物实验室（MBL）在伍兹霍尔，MA，美国。这两个中心共同为非洲爪蟾研究社区提供资源和支持。EXRC和NXR作为储备中心，获取、生产、维护和分发突变体、近交系和转基因X。laevis和X.热带线独立地，EXRC是非洲爪蟾cDNA、fosmids和抗体的储存库;它还提供英国境内的卵母细胞和野生型青蛙。NXR将通过举办小型课程和研讨会以及为研究人员提供在MBL执行短期项目的空间来提供研究培训和促进知识交流，从而补充这些服务。EXRC和NXR将共同使研究人员能够通过为从研究生到经验丰富的PI的各个层次提供资源和专业知识来提高生产力。这两个中心还将使使用其他动物系统的研究人员能够利用非洲爪蟾独特的实验特征来补充他们的研究。
Xenopus is an essential vertebrate model system for biomedical research that has contributed to important discoveries in many disciplines, including cell biology, molecular biology, physiology, developmental biology and neurobiology. However, unlike other model systems no central repository/stock center for Xenopus had been established until recently. Similar to mouse, zebrafish and fly communities, which have established stock centers, Xenopus researchers need to maintain and distribute rapidly growing numbers of inbred, mutant and transgenic frog strains, along with DNA and protein resources, and individual laboratories struggle to accomplish this efficiently. In the last five years two resource centers were founded to address this need: the European Xenopus Resource Center (EXRC) at the University of Portsmouth in England, and the National Xenopus Resource (NXR) at the Marine Biological Laboratory (MBL) in Woods Hole, MA, USA. These two centers work together to provide resources and support to the Xenopus research community. The EXRC and NXR serve as stock centers and acquire, produce, maintain and distribute mutant, inbred and transgenic X. laevis and X. tropicalis lines. Independently, the EXRC is a repository for Xenopus cDNAs, fosmids and antibodies; it also provides oocytes and wild type frogs within the UK. The NXR will complement these services by providing research training and promoting intellectual interchange through hosting minicourses and workshops and offering space for researchers to perform short-term projects at the MBL. Together the EXRC and NXR will enable researchers to improve productivity by providing resources and expertise to all levels, from graduate students to experienced PIs. These two centers will also enable investigators that use other animal systems to take advantage of Xenopus’ unique experimental features to complement their studies.
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影响因子：
16
作者：
Ben-Yehoyada M;Wang LC;Kozekov ID;Rizzo CJ;Gottesman ME;Gautier J
通讯作者：
Gautier J
影响因子：
11.4
作者：
Harland, Richard M.;Grainger, Robert M.
通讯作者：
Grainger, Robert M.
影响因子：
9.2
作者：
Horb, ME;Shen, CN;Slack, JMW
通讯作者：
Slack, JMW
影响因子：
1.5
作者：
Fish, Margaret B.;Nakayama, Takuya;Grainger, Robert M.
通讯作者：
Grainger, Robert M.
DOI：
10.1007/978-1-60327-019-9_4
发表时间：
2009-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Loeber, Jana;Pan, Fong Cheng;Pieler, Tomas
通讯作者：
Pieler, Tomas