Juvenile hormone-mediated reproduction induced by a sublethal concentration of imidacloprid in Cyrtorhinus lividipennis (Hemiptera: Miridae)
Juvenile hormone-mediated reproduction induced by a sublethal concentration of imidacloprid in Cyrtorhinus lividipennis (Hemiptera: Miridae)
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亚致死浓度的吡虫啉诱导Cyrtorhinus lividipennis（半翅目：Miridae）保幼激素介导的繁殖
DOI：
10.1016/j.aspen.2019.10.018
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发表时间：
2020-04
影响因子：
1.5
通讯作者：
Fang Liu
中科院分区：
文献类型：
--
作者：
Jun Zhu;Yao Li;Yingying Tang;Jianqi Liu;Yiyu Chen;Zhongxian Lv;Fang Liu
半翅目的捕食者，Cyrtorhinus lividipennis，以稻飞虱和叶蝉的卵和幼虫为食。我们以前证明，亚致死浓度的吡虫啉刺激C。青翅类考虑到保幼激素（JH）在昆虫繁殖中的重要作用，我们推测亚致死浓度的吡虫啉可能刺激了昆虫的繁殖。青翅凤蝶通过调节JH水平而获得。为了验证这一点，我们克隆了C。青翅凤蝶JH酸甲基转移酶（ClJHAMT）和JH酯酶（ClJHE），它们分别负责JH的生物合成和降解基因。然后，我们通过将dsRNA注射到C.结果表明，与对照组相比，羽化雌成虫卵黄蛋白原基因（ClVg）的表达量降低了88.8%，产卵量减少了41.5%。沉默ClJHE使ClVg mRNA表达增加275.0%，但不影响繁殖力。亚致死浓度的吡虫啉（LC 20）增加JH滴度在女性的35.3%和60.6%，分别在24和48小时后出现。在含有吡虫啉和dsJHAMT两者的处理中，与对照（吡虫啉+dsGFP）相比，CLJHAMT的沉默使成年雌性产生的卵的数量减少了21.4%。结果表明，亚致死浓度的吡虫啉可诱导C.通过JHAMT上调JH水平来调节青翅凤蝶的繁殖。这一发现为提高C.青翅目昆虫和杀虫剂在害虫管理中的应用。
The Hemipteran predator, Cyrtorhinus lividipennis, feeds on the eggs and nymphs of rice planthoppers and leafhoppers. We previously demonstrated that sublethal concentrations of imidacloprid stimulated the reproduction of C. lividipennis. Considering the essential roles of juvenile hormone (JH) in insect reproduction, we speculated that sublethal concentrations of imidacloprid may stimulate the reproduction of C. lividipennis by regulating JH level. To test this, we cloned C. lividipennis JH acid methyl transferase (ClJHAMT) and JH esterase (ClJHE), which are responsible for JH biosynthesis and degradation genes, respectively. We then knocked down ClJHAMT by injecting dsRNA into C. lividipennis nymphs and found that emerging female adults exhibited 88.8% lower expression of the vitellogenin gene (ClVg) and the number of eggs was reduced by 41.5% as compared with controls. Silencing ClJHE increased ClVg mRNA expression by 275.0% but did not affect fecundity. A sublethal concentration of imidacloprid (LC20) increased the JH titer in females by 35.3% and 60.6% at 24 and 48 h post-emergence, respectively. In treatments containing both imidacloprid and dsJHAMT, the silencing of CLJHAMT reduced the number of eggs produced by adult females by 21.4% as compared to the control (imidacloprid + dsGFP). Our results indicated that sublethal concentration of imidacloprid may induce C. lividipennis reproduction by upregulating JH level via JHAMT. The finding could provide valuable information for improved integration of C. lividipennis and insecticides in pest management.
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DOI：
10.1016/b978-012532104-4/50038-x
发表时间：
2002
期刊：
Hormones, brain and behavior
影响因子：
--
作者：
J. Ringo
通讯作者：
J. Ringo
影响因子：
--
作者：
Hui Wei;Juan Wang;Hongshan Li;H. Dai;Xiaohong Gu
通讯作者：
Hui Wei;Juan Wang;Hongshan Li;H. Dai;Xiaohong Gu
影响因子：
2.2
作者：
Liu, Shuhua;Yangb, Baojun;Liu, Zewen
通讯作者：
Liu, Zewen
影响因子：
2.8
作者：
Wang, Huai Yin;Yang, Yang;Zhu, Yu Cheng
通讯作者：
Zhu, Yu Cheng
选择用于生态工程的花蜜植物，以促进掠食性虫子Cyrtorhinus lividipennis（Heteroptera：Miridae）来促进水稻害虫的生物控制。
影响因子：
3.7
作者：
Zhu P;Lu Z;Heong K;Chen G;Zheng X;Xu H;Yang Y;Nicol HI;Gurr GM
通讯作者：
Gurr GM