Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells.
Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells.
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III 型转化生长因子-β 受体 RNA 干扰增强人间充质干细胞中转化生长因子-β 3 诱导的软骨形成信号传导
DOI：
10.1155/2018/4180857
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发表时间：
2018
影响因子：
4.3
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Zheng S;Zhou H;Chen Z;Li Y;Zhou T;Lian C;Gao B;Su P;Xu C
III型转化生长因子-β (TGF-β)受体（t -β riii）是TGF-β超家族的一种辅助受体，已知可结合TGF-βs并调节TGF-β信号传导。然而，t -β riii在TGF-β诱导的间充质干细胞（MSC）软骨形成中的调节作用尚未探讨。本研究探讨了t -β riii RNA干扰（RNAi）对TGF-β3诱导的人MSC （hMSC）软骨形成的影响及可能的信号机制。构建含有TβRIII小干扰RNA (siRNA) （SiTβRIII）或对照siRNA （SiNC）基因的慢病毒表达载体，并将其感染到hMSCs中。细胞在含TGF-β3的成软骨培养基或对照培养基中培养。与SiNC感染的细胞相比，TβRIII RNAi显著增强TGF-β3诱导的hMSCs成软骨分化、II型（TβRII）与I型（TβRI） TGF-β受体的比例以及Smad2/3的磷酸化水平。SB431542是一种TGF-β信号抑制剂，不仅抑制TβRIII RNAi刺激的TGF-β3介导的Smad2/3磷酸化，而且抑制TβRIII RNAi对TGF-β3诱导的软骨分化的作用。这些结果表明，t -β riii RNAi通过激活TGF-β/Smad2/3信号通路，增强TGF-β3诱导的hMSCs成软骨分化。这一发现指出了通过敲低TβRIII修饰MSCs作为未来基于细胞的软骨组织工程的有效策略的可能性。
The type III transforming growth factor-β (TGF-β) receptor (TβRIII), a coreceptor of the TGF-β superfamily, is known to bind TGF-βs and regulate TGF-β signaling. However, the regulatory roles of TβRIII in TGF-β-induced mesenchymal stem cell (MSC) chondrogenesis have not been explored. The present study examined the effect of TβRIII RNA interference (RNAi) on TGF-β3-induced human MSC (hMSC) chondrogenesis and possible signal mechanisms. A lentiviral expression vector containing TβRIII small interfering RNA (siRNA) (SiTβRIII) or a control siRNA (SiNC) gene was constructed and infected into hMSCs. The cells were cultured in chondrogenic medium containing TGF-β3 or control medium. TβRIII RNAi significantly enhanced TGF-β3-induced chondrogenic differentiation of hMSCs, the ratio of type II (TβRII) to type I (TβRI) TGF-β receptors, and phosphorylation levels of Smad2/3 as compared with cells infected with SiNC. An inhibitor of the TGF-β signal, SB431542, not only inhibited TβRIII RNAi-stimulated TGF-β3-mediated Smad2/3 phosphorylation but also inhibited the effects of TβRIII RNAi on TGF-β3-induced chondrogenic differentiation. These results demonstrate that TβRIII RNAi enhances TGF-β3-induced chondrogenic differentiation in hMSCs by activating TGF-β/Smad2/3 signaling. The finding points to the possibility of modifying MSCs by TβRIII knockdown as a potent future strategy for cell-based cartilage tissue engineering.
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影响因子：
3.3
作者：
Tazat K;Hector-Greene M;Blobe GC;Henis YI
通讯作者：
Henis YI
影响因子：
6
作者：
Tan AR;Hung CT
通讯作者：
Hung CT
影响因子：
4.8
作者：
Eickelberg, O;Centrella, M;Wells, RG
通讯作者：
Wells, RG
影响因子：
2.6
作者：
Albrecht, Christian;Hosiner, Stefanie;Nuernberger, Sylvia
通讯作者：
Nuernberger, Sylvia
外源硫酸乙酰肝素通过激活 TGF-β/Smad 信号传导增强人间充质干细胞中 TGF-β 3 诱导的软骨形成
影响因子：
4.3
作者：
Chen, Juan;Wang, Yongqian;Xu, Caixia
通讯作者：
Xu, Caixia