Insulin, epidermal growth factor and fibroblast growth factor elicit distinct patterns of protein tyrosine phosphorylation in BC3H1 cells.
Insulin, epidermal growth factor and fibroblast growth factor elicit distinct patterns of protein tyrosine phosphorylation in BC3H1 cells.
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胰岛素、表皮生长因子和成纤维细胞生长因子在 BC3H1 细胞中引发不同的蛋白质酪氨酸磷酸化模式。
DOI：
10.1016/0167-4889(90)90207-t
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Avruch,J
中科院分区：
文献类型：
--
作者：
Kyriakis,JM;Avruch,J
用抗磷酸酪氨酸抗体免疫印迹法检测小鼠肌样细胞系BC3H1对胰岛素、表皮生长因子（EGF）和成纤维细胞生长因子（FGF）响应时酪氨酸残基磷酸化的多肽。每一种配体都引发了一组特征的、大部分不重叠的多肽底物的酪氨酸磷酸化，按亚基mr、pI、亚细胞分离和吸附到凝集素（即凝集素- sepharose）柱上的行为进行分类。由单一配体引起的所有受刺激酪氨酸磷酸化的剂量-反应曲线是重叠的。相比之下，每个配体引发的反应的时间模式在刺激的开始速度和持续时间方面有所不同。对胰岛素的磷酸化反应几乎在瞬间达到最大值，并完全维持至少30分钟。在最初的15-60秒内，对EGF的响应稳步上升至峰值，此后逐渐下降。FGF刺激的磷酸化直到加入FGF后4分钟才被检测到，在接下来的30秒内突然上升到最大值，随后下降。在加入激素之前，将BC3H1细胞暴露于活性磷酯中，以不同的方式改变了对激素的反应。FGF反应被消除，EGF反应被部分抑制，而对胰岛素的反应不受影响。因此，胰岛素、EGF和FGF各自作用于单个细胞，介导一种特征的、基本上不重叠的多肽底物阵列的酪氨酸磷酸化，这表明这些受体酪氨酸激酶中的每一种都表现出根本不同的底物特异性。对每个配体反应的动力学和调控特性的差异也很明显，并反映了每个受体酪氨酸激酶原位作用的不同调控特性。
The polypeptides which are phosphorylated at tyrosine residues in the murine muscle-like cell line, BC3H1, in response to insulin, epidermal growth factor (EGF) and fibroblast growth factor (FGF) were detected by immunoblotting with antiphosphotyrosine antibodies. Each ligand elicited the tyrosine phosphorylation of a characteristic, largely nonoverlapping set of polypeptide substrates, as classified by subunitMr, pI, behavior on subcellular fractionation and adsorption to lectin (what germ agglutinin-Sepharose) columns. The dose-response curves for all stimulated tyrosine phosphorylations elicited by a single ligand were superimposable. By contrast, the temporal pattern of the responses elicited by each ligand differed in regard to speed of onset and persistence of the stimulation. Phosphorylation in response to insulin was maximal in a virtually instantaneous fashion and was fully maintained for at least 30 min. The response to EGF increased steadily over the initial 15–60 s to peak values, and fell progressively thereafter. FGF-stimulated phosphorylation was not detectable until 4 min after FGF addition, abruptly rose to maximal within the next 30 s, and declined subsequently. Exposure of BC3H1 cells to active phorbol esters prior to hormone addition altered the response to hormones in a differential fashion. FGF responses were abolished, EGF responses were partially inhibited, whereas the response to insulin was unaffected. Thus, acting on a single cell, insulin, EGF and FGF each mediate the tyrosine phosphorylation of a characteristic, largely nonoverlapping array of polypeptide substrates, indicating that each of these receptor tyrosine kinases exhibits a fundamentally distinct substrate specificity. Differences in the kinetic and regulatory properties of the response to each ligand are also apparent, and reflect the differing regulatory properties of each receptor tyrosine kinase acting in situ.
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DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
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通讯作者：
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DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tornqvist,HE;Avruch,J
通讯作者：
Avruch,J
影响因子：
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作者：
M. Wahl;T. Daniel;G. Carpenter
通讯作者：
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影响因子：
56.9
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通讯作者：
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影响因子：
64.5
作者：
A. Otte;P. Run;M. Heideveld;R. Driel;A. Durston
通讯作者：
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