Metabolic discrimination of select list agents by monitoring cellular responses in a multianalyte microphysiometer.
Metabolic discrimination of select list agents by monitoring cellular responses in a multianalyte microphysiometer.
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DOI：
10.3390/s90302117
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Cliffel DE
中科院分区：
文献类型：
--
作者：
Eklund SE;Thompson RG;Snider RM;Carney CK;Wright DW;Wikswo J;Cliffel DE
利用细胞作为复杂生物传感器的潜力，有望创造出灵敏和选择性的检测器，用于区分生物防御剂。在这里，我们提出了毒素检测，并建议歧视使用细胞的多分析物微生理计（MMP），能够同时测量通量的变化，在四个细胞外分析物（酸化率，葡萄糖摄取，氧摄取，乳酸生产）在实时。肉毒杆菌神经毒素A和蓖麻毒素与神经母细胞瘤细胞，丙甲霉素和炭疽保护性抗原与RAW巨噬细胞，霍乱毒素，毒蕈碱，2，4-二硝基苯酚和氟化钠与CHO细胞之间观察到不同的短期细胞反应。这些结果和在每种情况下观察到的暴露后动力学和代谢恢复表明基于细胞的检测器在复杂基质中区分特定分析物和化合物类别的有用性，并且还对试剂的细胞效应进行代谢推断。这可能对未知毒素的分类特别有价值。
Harnessing the potential of cells as complex biosensors promises the potential to create sensitive and selective detectors for discrimination of biodefense agents. Here we present toxin detection and suggest discrimination using cells in a multianalyte microphysiometer (MMP) that is capable of simultaneously measuring flux changes in four extracellular analytes (acidification rate, glucose uptake, oxygen uptake, and lactate production) in real-time. Differential short-term cellular responses were observed between botulinum neurotoxin A and ricin toxin with neuroblastoma cells, alamethicin and anthrax protective antigen with RAW macrophages, and cholera toxin, muscarine, 2,4-dinitro-phenol, and NaF with CHO cells. These results and the post exposure dynamics and metabolic recovery observed in each case suggest the usefulness of cell-based detectors to discriminate between specific analytes and classes of compounds in a complex matrix, and furthermore to make metabolic inferences on the cellular effects of the agents. This may be particularly valuable for classifying unknown toxins.
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影响因子：
4.5
作者：
Eklund, SE;Snider, RM;Cliffel, DE
通讯作者：
Cliffel, DE
DOI：
10.1073/pnas.75.7.3050
发表时间：
1978-01-01
影响因子：
11.1
作者：
GILL, DM;MEREN, R
通讯作者：
MEREN, R
影响因子：
3.2
作者：
Cao, CJ;Mioduszewski, RJ;Eldefrawi, AT
通讯作者：
Eldefrawi, AT
影响因子：
3.9
作者：
LORD, JM;WALES, R;ROBERTS, LM
通讯作者：
ROBERTS, LM
影响因子：
2.9
作者：
Landwojtowicz, E;Nervi, P;Seelig, A
通讯作者：
Seelig, A