Balancing act of a leading strand DNA polymerase-specific domain and its exonuclease domain promotes genome-wide sister replication fork symmetry.
Balancing act of a leading strand DNA polymerase-specific domain and its exonuclease domain promotes genome-wide sister replication fork symmetry.
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DOI：
10.1101/gad.350054.122
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发表时间：
2023-02-01
影响因子：
10.5
通讯作者：
Zhao, Xiaolan
中科院分区：
文献类型：
--
作者：
Meng, Xiangzhou;Claussin, Clemence;Regan-Mochrie, Gemma;Whitehouse, Iestyn;Zhao, Xiaolan
在这项研究中，Meng等人描述了酵母Pol2聚合酶的保守POPS和核酸外切酶结构域之间的拮抗作用及其在进行性DNA合成和姐妹叉对称性中的重要性。他们表明，由POPS突变引起的多种缺陷通过核酸外切酶失活而得到拯救，包括生长和DNA合成受损，基因组不稳定性以及对其他基因组维持因子的依赖，单分子数据显示，这种拯救源于允许姐妹复制叉以相等的速度进展。Pol2是芽殖酵母中的前导链DNA聚合酶。在这里，我们描述了其保守的POPS（Pol2家族特异性催化核心外围亚结构域）和核酸外切酶结构域之间的拮抗作用，以及这种拮抗作用在基因组复制中的重要性。我们发现，由POPS突变引起的多种缺陷，包括生长和DNA合成受损，基因组不稳定性，以及对其他基因组维持因子的依赖，都可以通过核酸外切酶失活来挽救。单分子数据显示，拯救源于允许姐妹复制叉以相同的速度进行。我们的数据表明Pol2的POPS和核酸外切酶结构域的平衡活性对于基因组复制和稳定性至关重要。
In this study, Meng et al. describe an antagonism between the conserved POPS and exonuclease domains of the yeast Pol2 polymerase and its importance in processive DNA synthesis and sister fork symmetry. They show that multiple defects caused by POPS mutations were rescued by exonuclease inactivation, including impaired growth and DNA synthesis, genome instability, and reliance on other genome maintenance factors, and single-molecule data revealed that the rescue stemmed from allowing sister replication forks to progress at equal rates. Pol2 is the leading-strand DNA polymerase in budding yeast. Here we describe an antagonism between its conserved POPS (Pol2 family-specific catalytic core peripheral subdomain) and exonuclease domain and the importance of this antagonism in genome replication. We show that multiple defects caused by POPS mutations, including impaired growth and DNA synthesis, genome instability, and reliance on other genome maintenance factors, were rescued by exonuclease inactivation. Single-molecule data revealed that the rescue stemmed from allowing sister replication forks to progress at equal rates. Our data suggest that balanced activity of Pol2's POPS and exonuclease domains is vital for genome replication and stability.
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DOI：
10.1073/pnas.1418334111
发表时间：
2014-10-28
影响因子：
11.1
作者：
Langston, Lance D.;Zhang, Dan;O'Donnell, Mike E.
通讯作者：
O'Donnell, Mike E.
影响因子：
10.5
作者：
Eaton, Matthew L.;Galani, Kyriaki;MacAlpine, David M.
通讯作者：
MacAlpine, David M.
影响因子：
16.8
作者：
Hogg, Matthew;Osterman, Pia;Johansson, Erik
通讯作者：
Johansson, Erik
真核铅和滞后链DNA聚合酶通过单独的机制加载到引物上，但在PCNA存在下具有可比的加工性。
影响因子：
14.9
作者：
Chilkova O;Stenlund P;Isoz I;Stith CM;Grabowski P;Lundström EB;Burgers PM;Johansson E
通讯作者：
Johansson E
真核生物的回复体如何实现快速有效的DNA复制。
影响因子：
16
作者：
Yeeles JTP;Janska A;Early A;Diffley JFX
通讯作者：
Diffley JFX