Generation and characterization of a Meflin-CreERT2 transgenic line for lineage tracing in white adipose tissue.
Generation and characterization of a Meflin-CreERT2 transgenic line for lineage tracing in white adipose tissue.
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DOI：
10.1371/journal.pone.0248267
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Tobe K
中科院分区：
文献类型：
--
作者：
Kuwano T;Izumi H;Aslam MR;Igarashi Y;Bilal M;Nishimura A;Watanabe Y;Nawaz A;Kado T;Ikuta K;Yamamoto S;Sasahara M;Fujisaka S;Yagi K;Mori H;Tobe K
Meflin（Islr）表达作为间充质干细胞的标志物已受到关注，但其功能仍在很大程度上未被探索。在这里，我们报告了Meflin-CreERT 2小鼠与CreERT 2下Meflin基因启动子插入标记Meflin表达细胞的遗传，从而使他们的谱系被跟踪。我们发现，在成年小鼠中，表达Meflin的谱系细胞存在于脂肪组织基质中，并已分化为成熟的脂肪细胞。这些细胞在高脂饮食负荷后的小鼠脂肪组织中构成冠状结构。冷刺激导致表达Meflin的谱系细胞分化成米色脂肪细胞。因此，Meflin-CreERT 2小鼠系是用于可视化和追踪表达Meflin的细胞谱系的有用的新工具。
Meflin (Islr) expression has gained attention as a marker for mesenchymal stem cells, but its function remains largely unexplored. Here, we report the generation of Meflin-CreERT2 mice with CreERT2 inserted under the Meflin gene promoter to label Meflin-expressing cells genetically, thereby enabling their lineages to be traced. We found that in adult mice, Meflin-expressing lineage cells were present in adipose tissue stroma and had differentiated into mature adipocytes. These cells constituted Crown-like structures in the adipose tissue of mice after high-fat diet loading. Cold stimulation led to the differentiation of Meflin-expressing lineage cells into beige adipocytes. Thus, the Meflin-CreERT2 mouse line is a useful new tool for visualizing and tracking the lineage of Meflin-expressing cells.
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影响因子：
29
作者：
Berry R;Jeffery E;Rodeheffer MS
通讯作者：
Rodeheffer MS
影响因子：
4.6
作者：
Horikawa S;Ishii Y;Hamashima T;Yamamoto S;Mori H;Fujimori T;Shen J;Inoue R;Nishizono H;Itoh H;Majima M;Abraham D;Miyawaki T;Sasahara M
通讯作者：
Sasahara M
影响因子：
29
作者：
Kajimura S;Spiegelman BM;Seale P
通讯作者：
Seale P
影响因子：
4.6
作者：
Maeda K;Enomoto A;Hara A;Asai N;Kobayashi T;Horinouchi A;Maruyama S;Ishikawa Y;Nishiyama T;Kiyoi H;Kato T;Ando K;Weng L;Mii S;Asai M;Mizutani Y;Watanabe O;Hirooka Y;Goto H;Takahashi M
通讯作者：
Takahashi M
影响因子：
8.8
作者：
Cattaneo, Paola;Mukherjee, Debanjan;Guimaraes-Camboa, Nuno
通讯作者：
Guimaraes-Camboa, Nuno