Fingerpick Blood-Based Nucleic Acid Testing on A USB Interfaced Device towards HIV self-testing.
Fingerpick Blood-Based Nucleic Acid Testing on A USB Interfaced Device towards HIV self-testing.
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DOI：
10.1016/j.bios.2022.114255
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发表时间：
2022-08-01
影响因子：
12.6
通讯作者：
中科院分区：
文献类型：
--
作者：
艾滋病毒自我检测是一种新兴的创新方法，它允许想要知道自己艾滋病毒状况的个人收集自己的样本，进行测试，并私下解释结果。现有的艾滋病毒自我检测方法依赖于快速诊断试验(RDT)来检测艾滋病毒-1/2抗体的存在，在窗口期内，这种抗体可能会漏掉相当大一部分无症状携带者。在这项工作中，我们提出了一个完全集成的核酸检测(NAT)设备，以简化使用100μL指刺全血进行艾滋病毒自我检测。该装置由即用型微流控试剂盒和超紧凑型NAT-on-USB分析仪组成。该检测只需用户简单的步骤，将手指刺伤的血液样本放入带有裂解缓冲液的采集管中，并将裂解产物加载到微流控芯片上，即可通过定制的图形用户界面(GUI)轻松读出检测结果。微流控色谱柱和分析仪可自动处理样品制备、纯化和实时逆转录环介导等温扩增(RT-LAMP)的复杂性。在约60分钟的周转时间内，在95%的置信度水平下，我们实现了214个病毒RNA拷贝/毫升全血的检测极限(LOD)。由于其易用性和高灵敏度，我们预计艾滋病毒NAT-on-USB设备将对寻求在暴露的早期阶段进行私人自我检测的高危人群特别有用。
HIV self-testing is an emerging innovative approach that allows individuals who want to know their HIV status to collect their own specimen, perform a test, and interpret the results privately. Existing HIV self-testing methods rely on rapid diagnostic tests (RDTs) to detect the presence of HIV-1/2 antibodies, which could miss a significant portion of asymptomatic carriers during the window period. In this work, we present a fully integrated nucleic acid testing (NAT) device towards streamlined HIV self-testing using 100 μL finger-prick whole blood. The device consists of a ready-to-use microfluidic reagent cartridge and an ultra-compact NAT-on-USB analyzer. The test requires simple steps from the user to drop the finger-prick blood sample into a collection tube with lysis buffer and load the lysate onto the microfluidic cartridge, and the testing result can be easily read out by a custom-built graphical user interface (GUI). The microfluidic cartridge and the analyzer automatically handle the complexity of sample preparation, purification, and real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP). With a turnaround time of ~60 min, we achieved a limit of detection (LoD) of 214 viral RNA copies/mL of whole blood at a 95% confidence level. Due to its ease of use and high sensitivity, we anticipate the HIV NAT-on-USB device would be particularly useful for the high-risk populations seeking private self-testing at the early stages of exposure.
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影响因子：
12.7
作者：
Curtis, Kelly A.;Rudolph, Donna L.;Owen, Michele
通讯作者：
Owen, Michele
影响因子：
4.6
作者：
Fidler S;Lewis H;Meyerowitz J;Kuldanek K;Thornhill J;Muir D;Bonnissent A;Timson G;Frater J
通讯作者：
Frater J
影响因子：
3.4
作者：
Beyzavi, Ali;Nguyen, Nam-Trung
通讯作者：
Nguyen, Nam-Trung
影响因子：
3.7
作者：
Mugo, Peter M.;Micheni, Murugi;Sanders, Eduard J.
通讯作者：
Sanders, Eduard J.
DOI：
10.1016/j.snb.2016.01.073
发表时间：
2016-06-28
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Liao SC;Peng J;Mauk MG;Awasthi S;Song J;Friedman H;Bau HH;Liu C
通讯作者：
Liu C