Increased chromosomal mobility after DNA damage is controlled by interactions between the recombination machinery and the checkpoint.
Increased chromosomal mobility after DNA damage is controlled by interactions between the recombination machinery and the checkpoint.
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DOI：
10.1101/gad.317966.118
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发表时间：
2018-09-01
影响因子：
10.5
通讯作者：
Rothstein R
中科院分区：
文献类型：
--
作者：
Smith MJ;Bryant EE;Rothstein R
In this study, Smith et al. investigated how cells modulate chromosome mobility in response to DNA damage. They show that global chromosome mobility is regulated by the Rad51 recombinase and its mediator, Rad52, and their findings indicate that interplay between recombination factors and the checkpoint restricts increased mobility until recombination proteins are assembled at damaged sites. During homologous recombination, cells must coordinate repair, DNA damage checkpoint signaling, and movement of chromosomal loci to facilitate homology search. In Saccharomyces cerevisiae, increased movement of damaged loci (local mobility) and undamaged loci (global mobility) precedes homolog pairing in mitotic cells. How cells modulate chromosome mobility in response to DNA damage remains unclear. Here, we demonstrate that global chromosome mobility is regulated by the Rad51 recombinase and its mediator, Rad52. Surprisingly, rad51Δ rad52Δ cells display checkpoint-dependent constitutively increased mobility, indicating that a regulatory circuit exists between recombination and checkpoint machineries to govern chromosomal mobility. We found that the requirement for Rad51 in this circuit is distinct from its role in recombination and that interaction with Rad52 is necessary to alleviate inhibition imposed by mediator recruitment to ssDNA. Thus, interplay between recombination factors and the checkpoint restricts increased mobility until recombination proteins are assembled at damaged sites.
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DOI：
10.1126/science.1219379
发表时间：
2012-09-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Cloud V;Chan YL;Grubb J;Budke B;Bishop DK
通讯作者：
Bishop DK
影响因子：
11.4
作者：
Herbert, Sebastien;Brion, Alice;Zimmer, Christophe
通讯作者：
Zimmer, Christophe
DOI：
10.1083/jcb.200810055
发表时间：
2009-06-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Burgess RC;Lisby M;Altmannova V;Krejci L;Sung P;Rothstein R
通讯作者：
Rothstein R
影响因子：
21.3
作者：
Lisby, M;Mortensen, UH;Rothstein, R
通讯作者：
Rothstein, R
DOI：
10.1073/pnas.121006298
发表时间：
2001-07-17
影响因子：
11.1
作者：
Lisby, M;Rothstein, R;Mortensen, UH
通讯作者：
Mortensen, UH