Correlation between phosphorylation of a 34,000-molecular-weight protein, pp60src-associated kinase activity, and tumorigenicity in transformed and revertant vole cells
Correlation between phosphorylation of a 34,000-molecular-weight protein, pp60src-associated kinase activity, and tumorigenicity in transformed and revertant vole cells
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转化和回复田鼠细胞中 34,000 分子量蛋白质的磷酸化、pp60src 相关激酶活性与致瘤性之间的相关性
DOI：
10.1128/mcb.4.1.212-215.1984
复制
发表时间：
1984
影响因子：
5.3
通讯作者：
A. Faras
中科院分区：
文献类型：
--
作者：
J. Nawrocki;A. Lau;A. Faras
在三种类型的劳斯肉瘤病毒感染的田鼠细胞中比较了34，000分子量（34K）细胞蛋白的磷酸化，该蛋白据称是禽逆转录病毒pp60src相关蛋白激酶活性的底物：完全转化的细胞，外观形态正常但保留其致瘤潜力的部分回复突变体，以及在所有参数（包括缺乏致瘤性）上与正常田鼠细胞相似的完全回复突变体。尽管在所有三种细胞类型中存在类似量的34K蛋白，但在完全回复突变细胞类型中34K蛋白的磷酸化显著降低。酪氨酸残基磷酸化减少。
The phosphorylation of a 34,000-molecular-weight (34K) cell protein, purported to be a substrate of the avian retrovirus pp60src-associated protein kinase activity, was compared in three types of Rous sarcoma virus-infected vole cells: fully transformed cells, partial revertants which are morphologically normal in appearance but retain their tumorigenic potential, and full revertants which are similar to normal vole cells in all parameters including a lack of tumorigenicity. Although similar amounts of 34K protein are present in all three cell types, phosphorylation of the 34K protein was significantly reduced in the full revertant cell type. The reduced phosphorylation occurred at the tyrosine residue.
DOI：
10.1073/pnas.76.10.5212
发表时间：
1979-10
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
K. Radke;G. Martin
通讯作者：
K. Radke;G. Martin
酪氨酸蛋白激酶细胞靶标的鉴定和表征。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cooper,JA;Hunter,T
通讯作者：
Hunter,T