How reliable are sino-nasal cell lines for studying the pathophysiology of chronic rhinosinusitis?
How reliable are sino-nasal cell lines for studying the pathophysiology of chronic rhinosinusitis?
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DOI：
10.1177/0003489414565003
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Fisher AJ
中科院分区：
文献类型：
--
作者：
Ball SL;Suwara MI;Borthwick LA;Wilson JA;Mann DA;Fisher AJ
充分表征的细胞系是研究人类疾病病理生理学的有用科学工具。慢性鼻窦炎 (CRS) 是一种非常常见的疾病，尽管 CRS 细胞系的数量有限，而且数据显示它们与原代细胞的相似程度也有限。使用美国典型培养物保藏中心 (ATCC) 和欧洲细胞培养物保藏中心 (ECACC) 搜索可用的人类细胞系。培养鉴定出的细胞，并通过染色和免疫组织化学染色以及 ELISA 进行表征，以评估它们对常见的、与疾病相关的炎症刺激的反应。经过仔细表型分析的 CRS 患者是在知情同意的情况下招募的。收获、培养原代鼻上皮细胞（PNEC）刷片，并与可用的细胞系进行比较。搜索确定了 1 种相关的 CRS 鼻鼻细胞系 RPMI 2650。培养的 PNEC 显示上皮标记物的强表达，而间充质标记物呈阴性。然而，RPMI 2650 细胞显示出非典型的混合上皮/间质表型。当受到促炎配体刺激时，PNEC 以剂量依赖性方式做出反应，而 RPMI 2650 细胞的反应有限。用于研究 CRS 病理生理学的细胞系的数量和可用性大大低估了疾病负担。此外，唯一的市售细胞系似乎与原代患者来源的细胞具有不同的表型和行为。进一步可重复的细胞系的开发将有利于我们对 CRS 的理解。
Well-characterized cell lines represent useful scientific tools to study the pathophysiology of human disease. Chronic rhinosinusitis (CRS) is a very common condition, though the number of CRS cell lines is limited, as are data showing how closely they resemble primary cells. Searches for available human cell lines were performed using the American Type Culture Collection (ATCC) and European Collection of Cell Cultures (ECACC). Identified cells were cultured and characterized with tinctorial and immunohistochemical staining and ELISA to assess their response to common, disease-relevant inflammatory stimuli. Carefully phenotyped CRS patients were recruited with informed consent. Primary nasal epithelial cell (PNEC) brushings were harvested, cultured, and compared to the available cell lines. Searches identified 1 relevant CRS sino-nasal cell line, RPMI 2650. Cultured PNECs showed strong expression of epithelial markers while being negative for mesenchymal markers. However, RPMI 2650 cells show an atypical mixed epithelial/mesenchymal phenotype. When stimulated by pro-inflammatory ligands, PNECs responded in a dose-dependent manner, whereas RPMI 2650 cells showed limited response. The number and availability of cell lines to study the pathophysiology of CRS greatly underrepresent the disease burden. Additionally, the sole commercially available cell line appears to have a different phenotype and behavior to primary patient-derived cells. The development of further reproducible cell lines would be beneficial in our understanding of CRS.
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影响因子：
2.6
作者：
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