Molecular evidence for natural hybridization in the mangrove fern genus Acrostichum.
Molecular evidence for natural hybridization in the mangrove fern genus Acrostichum.
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红树蕨属 Acrostichum 自然杂交的分子证据
DOI：
10.1186/1471-2229-13-74
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发表时间：
2013-05-01
影响因子：
5.3
通讯作者：
Zhou R
中科院分区：
文献类型：
--
作者：
Zhang R;Liu T;Wu W;Li Y;Chao L;Huang L;Huang Y;Shi S;Zhou R
自然杂交在蕨类植物中普遍存在，在蕨类植物的进化和物种形成中起着重要作用。在印度西太平洋地区，红树蕨类植物属acrostichum由两个主要的同域物种组成，A。aureumandA。speciosum。虽然以前没有种间杂交的报道，但我们首次在广东和海南发现了一些形态介于两者之间的个体，怀疑是杂种。在这项研究中，我们的目的是验证自然杂交的假设。aureumandA。广东和海南使用三个低拷贝核基因。在确定杂交状态后，利用叶绿体基因间间隔来推测杂交方向。此外，我们还研究了这些分类的孢子形状和萌发情况。aureumandA。三种核基因均表现出低水平的多态性；然而，它们在这些位点上分离得很好。在这两个位置，每个假定的杂交个体在两种表现出固定差异的所有位点的色谱上都表现出可加性。三个核基因的单倍型分析表明，每个杂种个体具有两个单倍型，与a的单倍型相匹配。aureumandA。分别speciosum。对叶绿体trnv - trnm区域的测序结果表明。aureumdiffered从。标本中有11个核苷酸替换和3个缺失（插入/缺失），所有样本与a具有相同的序列。speciosum。相比。aureumandA。假定的杂交种Speciosum孢子发芽率明显降低。结论三个核基因的序列数据为自然杂交提供了有力的证据。aureumandA。所有的杂交个体都可能是f1。杂化是单向的。基于叶绿体DNA母系遗传的假设，拟种为杂交亲本。人为干扰对红树生境的影响可能有利于红树属杂交植物的形成。
BackgroundNatural hybridization is prevalent in ferns, and plays an important role in fern evolution and speciation. In the Indo West-Pacific region, the mangrove fern genusAcrostichumconsists of two largely sympatric species,A. aureumandA. speciosum. Although there has been no report of interspecific hybridization before, we found some individuals morphologically intermediate between them in Guangdong and Hainan, China, for the first time, which were suspected to be hybrids. In this study, we aimed to test the hypothesis of natural hybridization betweenA. aureumandA. speciosumin Guangdong and Hainan using three low-copy nuclear genes. A chloroplast intergenic spacer was used to infer the hybridization direction once the hybrid status was confirmed. In addition, we examined spore shapes and germination for these taxa.ResultsBothA. aureumandA. speciosumshowed a low level of polymorphism at all three nuclear genes; however, they were well separated at these loci. At both locations, each individual of the putative hybrid showed additivity in chromatograms at all sites where the two species showed fixed differences. Haplotype analysis at all three nuclear genes indicated that each individual of the putative hybrid possessed two haplotypes, matching with those ofA. aureumandA. speciosum, respectively. Sequencing of the chloroplasttrnV-trnM regions showed thatA. aureumdiffered fromA. speciosumby eleven nucleotide substitutions and three indels (insertions/deletions), and all sampled individuals of the putative hybrid had the identical sequences withA. speciosum.Compared withA. aureumandA. speciosum, the putative hybrid had much reduced spore germination rate.ConclusionsSequence data of the three nuclear genes provide compelling evidence for natural hybridization betweenA. aureumandA. speciosum, and all the hybrid individuals are likely F1s. The hybridization is unidirectional andA. speciosumis the maternal parent of the hybrid based on the assumption of maternal inheritance of chloroplast DNA. Human disturbance on mangrove habitats may facilitate the establishment of hybrids ofAcrostichum.
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影响因子：
1.6
作者：
Dai, Seping;Wu, Wei;Zhou, Renchao
通讯作者：
Zhou, Renchao
影响因子：
4.9
作者：
Ebihara, Atsushi;Matsumoto, Sadamu;Ito, Motomi
通讯作者：
Ito, Motomi
影响因子：
3
作者：
GASTONY, GJ;YATSKIEVYCH, G
通讯作者：
YATSKIEVYCH, G
影响因子：
3.7
作者：
de Groot GA;During HJ;Maas JW;Schneider H;Vogel JC;Erkens RH
通讯作者：
Erkens RH
影响因子：
0.8
作者：
ADAMS, DC;TOMLINSON, PB
通讯作者：
TOMLINSON, PB