Guided Homing of Cells in Multi-Photon Microfabricated Bioscaffolds.
Guided Homing of Cells in Multi-Photon Microfabricated Bioscaffolds.
复制标题
多光子微生物生物镜托管中细胞的指导归巢。
DOI：
10.1002/adhm.201600082
复制
发表时间：
2016-05
影响因子：
10
通讯作者：
Yanik MF
中科院分区：
文献类型：
--
作者：
Skylar-Scott MA;Liu MC;Wu Y;Dixit A;Yanik MF
Tissues contain exquisite vascular microstructures, and patterns of chemical cues for directing cell migration, homing, and differentiation for organ development and function. 3D microfabrication by multi-photon photolithography is a flexible, high-resolution tool for generating 3D bioscaffolds. However, the combined fabrication of scaffold microstructure simultaneously with patterning of cues to create both geometrically and chemically defined microenvironments remains to be demonstrated. Here, we present a high-speed method for micron-resolution fabrication of scaffold microstructure and patterning of protein cues simultaneously using native scaffold materials. By the simultaneous microfabrication of arbitrary microvasculature geometries, and patterning selected regions of the microvasculature with the homing ligand P-selectin, we demonstrate adhesion, rolling, and selective homing of cells in defined 3D regions. This novel ability to rapidly generate high-resolution geometries replete with patterned cues at high speed enables the construction of biomimetic microenvironments for complex 3D assays of cell behavior.
登录
查看更多内容
影响因子：
2.8
作者：
Lee, Vivian K.;Lanzi, Alison M.;Ngo, Haygan;Yoo, Seung-Schik;Vincent, Peter A.;Dai, Guohao
通讯作者：
Dai, Guohao
影响因子：
14
作者：
Lee, Soo-Hong;Moon, James J.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
9
作者：
Koroleva, Anastasia;Gittard, Shaun;Chichkov, Boris
通讯作者：
Chichkov, Boris
影响因子：
3.9
作者：
Au, V;Madison, SA
通讯作者：
Madison, SA
影响因子：
41.2
作者：
通讯作者：
--