Generation of Multi-Scale Vascular Network System within 3D Hydrogel using 3D Bio-Printing Technology.
Generation of Multi-Scale Vascular Network System within 3D Hydrogel using 3D Bio-Printing Technology.
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DOI：
10.1007/s12195-014-0340-0
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发表时间：
2014-09
影响因子：
2.8
通讯作者：
Dai, Guohao
中科院分区：
文献类型：
--
作者：
Lee, Vivian K.;Lanzi, Alison M.;Ngo, Haygan;Yoo, Seung-Schik;Vincent, Peter A.;Dai, Guohao
尽管3D生物打印技术在创建具有多种细胞类型和基质的复杂组织方面具有巨大潜力，但由于缺乏血管灌注，在打印后保持厚组织结构对组织生长和成熟的活力具有挑战性。灌注毛细血管网络可以解决这个问题；然而，由于分配技术的时间和空间分辨率的限制，使用现有技术在单细胞水平上构建完整的毛细管网络几乎是不可能的。为了解决血管化问题，我们开发了一种3D打印方法来构建更大（管腔尺寸~1mm）的流体血管通道，并通过自然成熟过程创建相邻的毛细血管网络，从而提供了将毛细血管网络连接到大灌注血管通道的可行解决方案。在我们的模型中，微血管床在两个大的流体血管之间形成，然后通过从大通道边缘发芽的血管生成连接到血管。我们的生物打印技术在工程血管化厚组织和血管壁龛方面具有巨大潜力，因为血管通道是同时创建的，同时细胞和基质以所需的 3D 图案打印在通道周围。
Although 3D bio-printing technology has great potential in creating complex tissues with multiple cell types and matrices, maintaining the viability of thick tissue construct for tissue growth and maturation after the printing is challenging due to lack of vascular perfusion. Perfused capillary network can be a solution for this issue; however, construction of a complete capillary network at single cell level using the existing technology is nearly impossible due to limitations in time and spatial resolution of the dispensing technology. To address the vascularization issue, we developed a 3D printing method to construct larger (lumen size of ~1mm) fluidic vascular channels and to create adjacent capillary network through a natural maturation process, thus providing a feasible solution to connect the capillary network to the large perfused vascular channels. In our model, microvascular bed was formed in between two large fluidic vessels, and then connected to the vessels by angiogenic sprouting from the large channel edge. Our bio-printing technology has a great potential in engineering vascularized thick tissues and vascular niches, as the vascular channels are simultaneously created while cells and matrices are printed around the channels in desired 3D patterns.
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影响因子：
3
作者：
Langer, RS;Vacanti, JP
通讯作者：
Vacanti, JP
影响因子：
14
作者：
Lee, Wonhye;Debasitis, Jason Cushing;Yoo, Seung-Schik
通讯作者：
Yoo, Seung-Schik
分子机制和血管生成的临床应用。
影响因子：
64.8
作者：
Carmeliet, Peter;Jain, Rakesh K.
通讯作者：
Jain, Rakesh K.
影响因子：
2.1
作者：
Davis, George E.;Koh, Wonshill;Stratman, Amber N.
通讯作者：
Stratman, Amber N.
影响因子：
--
作者：
Fidkowski, C;Kaazempur-Mofrad, MR;Wang, YD
通讯作者：
Wang, YD