The melanopic sensitivity function accounts for melanopsin-driven responses in mice under diverse lighting conditions.
The melanopic sensitivity function accounts for melanopsin-driven responses in mice under diverse lighting conditions.
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DOI：
10.1371/journal.pone.0053583
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Lucas RJ
中科院分区：
文献类型：
--
作者：
Brown TM;Allen AE;al-Enezi J;Wynne J;Schlangen L;Hommes V;Lucas RJ
除了视杆细胞和视锥细胞之外，哺乳动物的感光还延伸到表达感光色素黑视蛋白的第三种视网膜细胞类型。这种新型视蛋白的影响范围广泛，从瞳孔和昼夜节律反应到亮度感知，但量化光生物效应的既定方法并不能充分考虑黑视蛋白敏感性。我们最近提出了一种新的指标，即黑素敏感度函数（VZλ）来解决这一缺陷。在这里，我们根据文献中确定的其适用性的潜在障碍或与明显的实际效益相关的各种测试进一步验证了这一新措施。使用电生理学方法和瞳孔测量法，最初在无杆+无锥小鼠中，我们的数据表明，在非常广泛的不同条件下（包括在具有高度发散光谱内容的刺激之间切换），VZλ函数可以准确预测黑视蛋白依赖性反应的灵敏度。我们进一步表明，VZλ 提供了对具有功能性视杆细胞和视锥细胞的小鼠视觉反应的至少一方面的光谱敏感性的最佳可用描述：外侧膝状体核的强直放电活动。总之，这些数据使 VZλ 成为一种重要的光测量新方法，具有广泛的实用性。
In addition to rods and cones, photoreception in mammals extends to a third retinal cell type expressing the photopigment melanopsin. The influences of this novel opsin are widespread, ranging from pupillary and circadian responses to brightness perception, yet established approaches to quantifying the biological effects of light do not adequately account for melanopsin sensitivity. We have recently proposed a novel metric, the melanopic sensitivity function (VZλ), to address this deficiency. Here, we further validate this new measure with a variety of tests based on potential barriers to its applicability identified in the literature or relating to obvious practical benefits. Using electrophysiogical approaches and pupillometry, initially in rodless+coneless mice, our data demonstrate that under a very wide range of different conditions (including switching between stimuli with highly divergent spectral content) the VZλ function provides an accurate prediction of the sensitivity of melanopsin-dependent responses. We further show that VZλ provides the best available description of the spectral sensitivity of at least one aspect of the visual response in mice with functional rods and cones: tonic firing activity in the lateral geniculate nuclei. Together, these data establish VZλ as an important new approach for light measurement with widespread practical utility.
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影响因子：
--
作者：
HARRINGTON, RE
通讯作者：
HARRINGTON, RE
影响因子：
9.8
作者：
Brown TM;Gias C;Hatori M;Keding SR;Semo M;Coffey PJ;Gigg J;Piggins HD;Panda S;Lucas RJ
通讯作者：
Lucas RJ
影响因子：
3.5
作者：
Rollag, Mark D.
通讯作者：
Rollag, Mark D.
影响因子：
4.8
作者：
Sexton, Timothy J.;Golczak, Marcin;Van Gelder, Russell N.
通讯作者：
Van Gelder, Russell N.
影响因子：
5.5
作者：
Brown, Timothy M.;Wynne, Jonathan;Lucas, Robert J.
通讯作者：
Lucas, Robert J.