Identification of genes differentially expressed during embryogenesis in Brassica campestris L.
Identification of genes differentially expressed during embryogenesis in Brassica campestris L.
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甘蓝胚胎发生过程中差异表达基因的鉴定。
DOI：
10.1007/s11738-011-0867-9
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发表时间：
2011-10
影响因子：
2.6
通讯作者：
Wang JB
中科院分区：
文献类型：
--
作者：
Li WL;Zhao Q;Tao P;Wang JB
利用cDNA-AFLP技术分析了甘蓝型油菜胚胎发生过程中的基因表达。整个胚胎发育过程分为6个胚胎期。从油菜胚胎发生过程中共获得4267条条带，共筛选出216个在6个胚胎发生阶段差异表达的转录本衍生片段(TDF)进行测序。通过对BLASTN和BLASTX的搜索，204个TDF大部分与拟南芥和甘蓝型油菜同源，其余12个TDF与其他植物的基因不匹配。结果，178个TDF与高等植物中编码已知或推测蛋白的基因匹配，26个TDF与未知基因同源。此外，通过RT-PCR验证了筛选出的差异表达的TDFs的子集，并显示了TDFs的相对转录丰度。白菜型油菜胚胎发生过程中表达了一些胚胎特异基因。许多TDF的表达谱与拟南芥相关基因的表达谱显示出相似性。对这些基因在胚胎发育中的潜在作用进行了讨论。
Gene expression in embryogenesis of Brassica campestris L. is analyzed using cDNA-AFLP technique. The whole embryogenesis was divided into six embryonic stages. In total, 4,267 bands were obtained from embryogenesis of B. campestris L. A total of 216 transcript-derived fragments (TDFs), differentially expressed in six stages of embryogenesis, were selected for sequencing. By searching BLASTN and BLASTX, most of 204 TDFs were homologous to Arabidopsis thaliana and Brassica napus sequences, and the rest of the 12 TDFs did not match with other plants’ genes. As a result, 178 TDFs are matched with genes encoding either known or putative proteins in higher plants, and 26 TDFs are homologous to unknown genes. Besides, a selected subset of differentially expressed TDFs is confirmed by RT-PCR, and relative transcript abundance of TDFs was shown. Some embryo-specific genes are expressed in embryogenesis of B. campestris. Expression profiles of many TDFs show similarity to those of the relevant Arabidopsis genes. The potential roles of these genes in embryo development are discussed.
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影响因子：
2.6
作者：
P. Zhao;Fei Liu;G. Zheng;Heng Liu
通讯作者：
P. Zhao;Fei Liu;G. Zheng;Heng Liu
影响因子：
4.9
作者：
Chen, GH;Huang, LT;Chen, SCG
通讯作者：
Chen, SCG
影响因子：
7.2
作者：
Bachem, CWB;vanderHoeven, RS;Visser, RGF
通讯作者：
Visser, RGF
DOI：
10.1073/pnas.1003530107
发表时间：
2010-05-04
影响因子：
11.1
作者：
Le, Brandon H.;Cheng, Chen;Goldberg, Robert B.
通讯作者：
Goldberg, Robert B.
影响因子：
7.2
作者：
Chiwocha, SDS;Cutler, AJ;Kermode, AR
通讯作者：
Kermode, AR