Kinesin-12 influences axonal growth during zebrafish neural development.
Kinesin-12 influences axonal growth during zebrafish neural development.
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Kinesin-12 影响斑马鱼神经发育过程中的轴突生长。
DOI：
10.1002/cm.21193
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发表时间：
2014-10
期刊：
影响因子：
2.9
通讯作者：
Liu, Mei
中科院分区：
文献类型：
--
作者：
Xu, Man;Liu, Dong;Dong, Zhangji;Wang, Xin;Wang, Xueqian;Liu, Yan;Baas, Peter W.;Liu, Mei
运动蛋白-12（也称为KIF15）是一种基于微管的运动蛋白，以其在细胞分裂中的作用而闻名。我们之前报道过，激酶12在发育终有丝分裂后神经元中强烈表达，随着神经元成熟，其表达水平下降。我们发现，在实验中，如果kinesin-12被耗尽，培养的啮齿动物神经元轴突生长得更快更长，这使我们得出结论，kinesin-12在调节轴突生长中起作用。在这里，我们用斑马鱼来探索这些结果是否适用于体内系统，以及它们是否适用于不同种类的脊椎动物。全载原位杂交，在2细胞期和k细胞期均可检测到激酶-12 mRNA。在受精后5.3和8 h，外胚层观察到激酶-12 mRNA的杂交信号。从14到36hpf，信号已经扩展到中枢神经系统。在60 hpf时，杂交信号集中在脑内。受精后5 d，激酶12表达降低。通过向Tg（huC:egfp）或Tg（hb9:gfp）斑马鱼胚胎注射一种剪接阻断的morpholino，使酪氨酸-12基因敲低导致生长迅速的轴突明显变长，分支减少。Kinesin-12过表达导致轴突比对照组短。这些结果与我们之前对使用原代培养进行实验操作的啮齿动物的观察结果一致，并表明激酶-12作为轴突发育调节剂的关键作用。
Kinesin-12 (also called KIF15) is a microtubule-based motor protein best known for its role in cell division. We previously reported that kinesin-12 is robustly expressed in developing terminally post-mitotic neurons, with levels diminishing as neurons reach maturity. We found that axons of cultured rodent neurons grow faster and longer if kinesin-12 is experimentally depleted, leading us to conclude that kinesin-12 plays a role in modulating axonal growth. Here we used zebrafish to explore whether these results apply to an in vivo system and whether they apply across different kinds of vertebrates. In whole mount in situ hybridization, kinesin-12 mRNA was detectable at 2-cell and 1K-cell stages. At 5.3 and 8 hours post-fertilization (hpf), hybridization signal for kinesin-12 mRNA was observed in the ectoderm. From 14 to 36 hpf, the signal had expanded to the central nervous system. At 60 hpf, the hybridization signal was concentrated in the brain. After 5 days post-fertilization, kinesin-12 expression was reduced. Kinesin-12 knockdown resulted in notably longer fast-growing axons with fewer branches by injection of a splice-blocking morpholino into Tg(huC:egfp) or Tg(hb9:gfp) zebrafish embryos. Kinesin-12 overexpression resulted in shorter axons than controls. These results are consistent with our previous observations on rodents using primary cultures for the experimental manipulations, and suggest a key role of kinesin-12 as a modulator of axonal development.
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影响因子：
4.5
作者：
Pramparo T;Libiger O;Jain S;Li H;Youn YH;Hirotsune S;Schork NJ;Wynshaw-Boris A
通讯作者：
Wynshaw-Boris A
DOI：
10.1107/s1399004713028721
发表时间：
2014-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Klejnot M;Falnikar A;Ulaganathan V;Cross RA;Baas PW;Kozielski F
通讯作者：
Kozielski F
影响因子：
7.7
作者：
Drechsler H;McHugh T;Singleton MR;Carter NJ;McAinsh AD
通讯作者：
McAinsh AD
影响因子：
9.2
作者：
Vanneste, David;Takagi, Masatoshi;Vernos, Isabelle
通讯作者：
Vernos, Isabelle
影响因子：
1.5
作者：
Sato, T;Takahoko, M;Okamoto, H
通讯作者：
Okamoto, H