Remarkable metabolic reorganization and altered metabolic requirements in frog metamorphic climax.
Remarkable metabolic reorganization and altered metabolic requirements in frog metamorphic climax.
复制标题
青蛙变态高潮期间显着的代谢重组和代谢需求的改变
DOI：
10.1186/s12983-020-00378-6
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发表时间：
2020
影响因子：
2.8
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Zhu W;Chang L;Zhao T;Wang B;Jiang J
变质的高潮是导致向陆地栖息地过渡的形态和功能变化的两栖动物的关键阶段。贮存。
我们使用转录组学和代谢组学的组合方法来研究自然和T3驱动的变质的高潮，而Rana Omeimontis ta则是息肉燃料的蛋白酸和脂肪酸的catib，catib catib和fatib catib and catib和fatib catib andim catib and catib catib and catib catib and catib intabiits catib and catib catib and catib catib and catib andim， ）与能量代谢一起上调（TCA循环和氧化磷酸化），而碳水化合物和脂质分解代谢（糖酵解，戊糖磷酸盐途径（PPP）和β-氧化）改善了肝糖原磷酸化和糖化作用。在尾部，糖酵解，β氧化和跨跨都被下调，通过同步完成能量生产和消耗的下调。 PHIC高潮。讨论了这些代谢调整及其潜在调节机制的重要性。
在分子水平上，氨基酸在变质水平上的能量策略和变质的高潮中的需求增加了碳水化合物在变质中的贡献。 ，，，，保护和两栖动物的发展研究。
BackgroundMetamorphic climax is the crucial stage of amphibian metamorphosis responsible for the morphological and functional changes necessary for transition to a terrestrial habitat. This developmental period is sensitive to environmental changes and pollution. Understanding its metabolic basis and requirements is significant for ecological and toxicological research.Rana omeimontistadpoles are a useful model for investigating this stage as their liver is involved in both metabolic regulation and fat storage.ResultsWe used a combined approach of transcriptomics and metabolomics to study the metabolic reorganization during natural and T3-driven metamorphic climax in the liver and tail ofRana omeimontistadpoles. The metabolic flux from the apoptotic tail replaced hepatic fat storage as metabolic fuel, resulting in increased hepatic amino acid and fat levels. In the liver, amino acid catabolism (transamination and urea cycle) was upregulated along with energy metabolism (TCA cycle and oxidative phosphorylation), while the carbohydrate and lipid catabolism (glycolysis, pentose phosphate pathway (PPP), and β-oxidation) decreased. The hepatic glycogen phosphorylation and gluconeogenesis were upregulated, and the carbohydrate flux was used for synthesis of glycan units (e.g., UDP-glucuronate). In the tail, glycolysis, β-oxidation, and transamination were all downregulated, accompanied by synchronous downregulation of energy production and consumption. Glycogenolysis was maintained in the tail, and the carbohydrate flux likely flowed into both PPP and the synthesis of glycan units (e.g., UDP-glucuronate and UDP-glucosamine). Fatty acid elongation and desaturation, as well as the synthesis of bioactive lipid (e.g., prostaglandins) were encouraged in the tail during metamorphic climax. Protein synthesis was downregulated in both the liver and tail. The significance of these metabolic adjustments and their potential regulation mechanism are discussed.ConclusionThe energic strategy and anabolic requirements during metamorphic climax were revealed at the molecular level. Amino acid made an increased contribution to energy metabolism during metamorphic climax. Carbohydrate anabolism was essential for the body construction of the froglets. The tail was critical in anabolism including synthesizing bioactive metabolites. These findings increase our understanding of amphibian metamorphosis and provide background information for ecological, evolutionary, conservation, and developmental studies of amphibians.
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影响因子：
--
作者：
Ichu TA;Han J;Borchers CH;Lesperance M;Helbing CC
通讯作者：
Helbing CC
影响因子：
4.8
作者：
Améen, C;Edvardsson, U;Oscarsson, J
通讯作者：
Oscarsson, J
DOI：
10.1073/pnas.93.18.9493
发表时间：
1996-09-03
影响因子：
11.1
作者：
Hochachka, PW;Buck, LT;Land, SC
通讯作者：
Land, SC
影响因子：
2.1
作者：
Gomez-Mestre, I.;Saccoccio, V. L.;Warkentin, K. M.
通讯作者：
Warkentin, K. M.
影响因子：
2.7
作者：
Buchholz, Daniel R.
通讯作者：
Buchholz, Daniel R.