Host gene expression modulated by Zika virus infection of human-293 cells.
Host gene expression modulated by Zika virus infection of human-293 cells.
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寨卡病毒感染人类-293细胞后宿主基因表达的调节。
DOI：
10.1016/j.virol.2020.09.007
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发表时间：
2021-01-02
期刊：
影响因子：
3.7
通讯作者：
Padmanabhan R
中科院分区：
文献类型：
--
作者：
Horibata S;Teramoto T;Vijayarangan N;Kuhn S;Padmanabhan R;Vasudevan S;Gottesman M;Padmanabhan R
HEK-293细胞系于1977年通过用剪切的腺病毒5型DNA转化原代人胚肾细胞而创建。先前的一项研究确定HEK-293细胞具有神经元标记物而不是肾脏标记物。在这项研究中，我们测试了寨卡病毒（ZIKV），一种嗜神经病毒，是否能够感染HEK-293细胞并在其中复制。我们显示，如通过间接免疫荧光（IFA）和定量逆转录酶-PCR（qRT-PCR）所示，用ZIKV感染的HEK-293细胞支持病毒复制。我们对ZIKV感染的HEK-293细胞和对照未感染的HEK-293细胞进行了RNA-seq分析，发现与未感染的细胞相比，在ZIKV感染的HEK-293细胞中差异转录的659个基因。结果表明，前10个差异转录和上调的基因参与抗病毒和炎症反应。通过qRT-PCR验证了7个上调基因，IFNL 1、DDX 58、CXCL 10、ISG 15、KCNJ 15、IFNIH 1和IFIT 2。总之，我们的研究结果表明，ZIKV感染通过影响宿主的抗病毒和炎症反应来改变宿主的基因表达。
The HEK-293 cell line was created in 1977 by transformation of primary human embryonic kidney cells with sheared adenovirus type 5 DNA. A previous study determined that the HEK-293 cells have neuronal markers rather than kidney markers. In this study, we tested the hypothesis whether Zika virus (ZIKV), a neurotropic virus, is able to infect and replicate in the HEK-293 cells. We show that the HEK-293 cells infected with ZIKV support viral replication as shown by indirect immunofluorescence (IFA) and quantitative reverse transcriptase-PCR (qRT-PCR). We performed RNA-seq analysis on the ZIKV-infected and the control uninfected HEK-293 cells and find 659 genes that are differentially transcribed in ZIKV-infected HEK-293 cells as compared to uninfected cells. The results show that the top 10 differentially transcribed and upregulated genes are involved in antiviral and inflammatory responses. Seven upregulated genes, IFNL1, DDX58 , CXCL10, ISG15, KCNJ15, IFNIH1, and IFIT2, were validated by qRT-PCR. Altogether, our findings show that ZIKV infection alters host gene expression by affecting their antiviral and inflammatory responses.
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影响因子：
5.8
作者：
Liberzon, Arthur;Subramanian, Aravind;Mesirov, Jill P.
通讯作者：
Mesirov, Jill P.
影响因子：
7.3
作者：
Hemann EA;Gale M Jr;Savan R
通讯作者：
Savan R
氯喹是 FDA 批准的药物，可预防寨卡病毒感染及其相关的小鼠先天性小头畸形
影响因子：
11.1
作者：
Li C;Zhu X;Ji X;Quanquin N;Deng YQ;Tian M;Aliyari R;Zuo X;Yuan L;Afridi SK;Li XF;Jung JU;Nielsen-Saines K;Qin FX;Qin CF;Xu Z;Cheng G
通讯作者：
Cheng G
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
影响因子：
23.9
作者：
Nowakowski TJ;Pollen AA;Di Lullo E;Sandoval-Espinosa C;Bershteyn M;Kriegstein AR
通讯作者：
Kriegstein AR