Progression of Nuclear Maturation and p34cdc2 Kinase Activity in Porcine Oocytes during In Vitro Culture in Different Media
Progression of Nuclear Maturation and p34cdc2 Kinase Activity in Porcine Oocytes during In Vitro Culture in Different Media
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不同培养基体外培养猪卵母细胞核成熟和p34cdc2激酶活性的进展
DOI：
10.1274/jmor.18.39
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Terada
中科院分区：
文献类型：
--
作者：
J. Ito;M. Shimada;T. Terada
在本研究中，以阐明不同的发育潜力的猪卵母细胞在两种培养基中成熟，mTCM 199和mNCSU 37，我们研究了在减数分裂成熟过程中的核状态和p34 cdc2激酶活性的变化的时间过程中的原因。mTCM199培养液中卵母细胞的p34 cdc2激酶活性较mNCSU 37培养液中的逐渐升高，mNCSU 37培养液中卵母细胞的p34 cdc2激酶活性从培养中期开始迅速升高，但两种培养液中的卵母细胞在培养末期的p34 cdc2激酶活性水平相似。在mTCM199中培养的卵母细胞经历GVBD，并且在培养期间比在mNCSU 37中培养的卵母细胞更早地达到MII期。此外，在mTCM199或mNCSU37中培养的MII卵母细胞在培养期结束时具有相似水平的p34cdc2激酶活性。因此可以得出结论，在不同培养基中成熟的卵母细胞发育能力的差异似乎与p34 cdc2激酶活性无关。
In the present study, to elucidate the cause of different developmental potential in porcine oocytes matured in two media, mTCM199 and mNCSU37, we examined the time course of changes in the nuclear states and p34cdc2 kinase activity of oocytes during meiotic maturation. The p34cdc2 kinase activity of oocytes cultured in mTCM199 gradually increased from earlier in the culture time periods than that of oocytes in mNCSU37, and that of oocytes in mNCSU37 rapidly increased from the middle of the culture period, but the oocytes in both media had similar levels of p34cdc2 kinase activity at the end of the culture period. Oocytes cultured in mTCM199 underwent GVBD and reached the MII stage earlier in the culture period than those in mNCSU37. Moreover, MII oocytes cultured in either mTCM199 or mNCSU37 had a similar level of p34cdc2 kinase activity at the end of the culture period. It was therefore concluded that the difference in developmental competence of oocytes matured in different media seems to be independent of p34cdc2 kinase activity.
影响因子：
3.6
作者：
Bin Wu;G. Ignotz;W. Bruce Currie;Xiangzhong Yang
通讯作者：
Bin Wu;G. Ignotz;W. Bruce Currie;Xiangzhong Yang
表皮生长因子、胰岛素样生长因子-I 和透析猪卵泡液对猪卵母细胞体外成熟的影响。
DOI：
10.1002/jez.1402660111
发表时间：
1993
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Reed,ML;Estrada,JL;Illera,MJ;Petters,RM
通讯作者：
Petters,RM